902 Harga | |
533 Catatan reproduksi | |
700 Entri Tambahan Nama Orang | |
336 Content Type | teks (rdacontent) |
264b Nama Penerbit | Official publicaation of the SEAMEO regional tropical medicine and public health network (TROPMED) |
710 Entri Tambahan Badan Korporasi | |
091 No. Panggil Setempat Majalah | 610 TSAJ 40:4 (2009) |
049 No. Barkod | |
852 Lokasi | Perpustakaan UI, Lantai 4, R. Koleksi Jurnal |
515 Penyimpangan/penomoran | |
338 Carrier Type | volume (rdacarier) |
490 Seri | |
270 Alamat Penerbit, Suplier | Faculty of Tropical Medicine, Mahidol University 420/6 Ratchawithi Road, Bangkok 10400, Thailand. |
590 Cat. Sumber Pengadaan Koleksi | |
053 No. Induk | |
321 Frekuensi lama | |
653 Kata Kunci | Falciparium; public health; southeast asean |
040 Sumber Pengatalogan | LibUI eng rda |
245 Judul Utama | The southeast asian journal of tropical medicine and public health |
264c Tahun Terbit | |
650 Subyek Topik | Medicine & health |
850 Lembaga Pemilik | Universitas Indonesia |
904b Pemeriksa Lembar Kerja | FakhriMubin |
520 Ringkasan/Abstrak/Intisari | This study aimed to demonstrate class switch recombination (CSR) in heavy chain expressing immunoglobulin G (IgG) and IgE in human B cells (CD20+CD27-) were cultured with crude P. falciparum antigen (cPfAg) and anti-CD40. on day 4 post-exposure, total RNA from B cells was prepared and the occurance of CSR from IgM to IgG and/or IgE was investigated by reverse transciption-polymerase chain reaction. Molecular markers to detect active CSR included enzyme activation-induced cytidine deaminase mRNA, y and e-germline transcripts (y,e-GLT), circle transcript (CT) and mature transcript (y and e-mRNA) expression. On day 7 and day 14 after exposure, levels of Igs in the culture supernatant were determined by enzyme-linked immunosorbent assay. Our findings showed that we could demonstrate cPfAg-stimulated B cells undergoing CSR by use of the expressed CSR markers and the increase in spesific IgG and IgE indicating the potential of this approach in the study of CSR in P. falciparum stimulated B cells. |
022 ISSN (R) | 01251562 |
525 Cat. suplemen/index/spec. issua | |
090 No. Panggil Setempat | 610 TSAJ |
061 Tanggal akhir diterima/langgan | |
500 Catatan Umum | |
337 Media Type | unmediated (rdamedia) |
100 Entri Utama Nama Orang | |
264a Kota Terbit | Bangkok, Thailand |
300 Deskripsi Fisik | |
904a Pengisi Lembar Kerja | |
856 Akses dan Lokasi Elektronik | |
246 Judul Alternatif | |
310 Frekuensi Terbit | |
041 Kode Bahasa | eng |
362 Tanggal Terbit Awal | |