| 700 Entri Tambahan Nama Orang | Wellyzar Sjamsuridzal, supervisor; Yokota, Akira, co-supervisor; Yasuhiro Igarashi, co-supervisor; Ariyanti Oetari, examiner; Is Helianti, examiner; Shuhei Yabe, examiner; Abinawanto, examiner |
| 001 Hak Akses (open/membership) | membership |
| 336 Content Type | text (rdacontent) |
| 710 Entri Tambahan Badan Korporasi | Fakultas Matematika dan Ilmu Pengetahuan Alam. Universitas Indonesia |
| 264b Nama Penerbit | Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia |
| 504 Catatan Bibliografi | pages 123-144 |
| 852 Lokasi | Perpustakaan UI |
| 049 No. Barkod | 07-22-08063446 |
| 338 Carrier Type | online resource (rdacarrier) |
| 590 Cat. Sumber Pengadaan Koleksi | |
| 903 Stock Opname | |
| Tahun Buka Akses | 2023 |
| 053 No. Induk | 07-22-08063446 |
| 653 Kata Kunci | Cisolok geothermal area; polyphasic taxonomy; thermophilic Actinobacteria |
| 040 Sumber Pengatalogan | LibUI eng rda |
| 245 Judul Utama | Studi Taksonomi Rare Actinobacteria Termofilik dan Penapisan Metabolit Sekunder Baru = Taxonomic Study of Rare Thermophilic Actinobacteria and Screening for Novel Secondary Metabolites |
| 650 Subyek Topik | Actinobacteria |
| 264c Tahun Terbit | 2022 |
| 850 Lembaga Pemilik | Universitas Indonesia |
| 520 Ringkasan/Abstrak/Intisari | The aims of this study are to provide data regarding the taxonomic study of thermophilic Actinobacteria based on 16S rRNA gene sequences, description, and assessment for secondary metabolite biosynthetic gene clusters (BGCs) in the genome of novel taxa, and its antibacterial activity. Thirty-one isolates of thermophilic Actinobacteria were isolated from soil samples in Cisolok geothermal area, West Java. The 16S rRNA gene sequence-similarity search against all related species was performed using EzTaxon-e database. The sequences of 31 isolates showed similarity to member of family Micromonosporaceae, Nocardiaceae, Pseudonocardiaceae, Streptomycetaceae, Streptosporangiaceae, and Thermomonosporaceae. Six isolates displayed high similarity to genera in the family Pseudonocardiaceae, and most closely related to the genus Thermotunica, T. guangxiensis AG2-7T with similarity values from 94.6 to 95.2%. Phenotypic features and phylogenetic data differentiated strain SL3-2-4T from members of the family Pseudonocardiaceae. Therefore, the strain SL3-2-4T is proposed as a representative of a novel species in a novel genus, Gandjariella thermophila gen. nov., sp. nov. The genome of SL3-2-4T contained 21 antiSMASH-identified secondary metabolite regions harboring BGCs. These BGCs were for polyketide synthase, non-ribosomal peptide synthase, and ribosomally synthesized and post-translationally modified peptide family clusters. Thirteen and five regions displayed low (4?35%) and no similarity with known BGCs for secondary metabolites, respectively. Screening for antibacterial activity showed that strains SL3-2-4T and SL3-2-7 on MM 2 medium solidified with gellan gum at 45°C for 14 days demonstrated inhibitory activity against all Gram-positive, but not Gram-negative bacteria. Strain SL3-2-10 on ISP 3 gellan gum medium incubated for seven-days only active against K. rhizophila NBRC 12078. The results indicated that novel taxa have the potential for the discovery of active secondary metabolites. |
| 904b Pemeriksa Lembar Kerja | |
| 090 No. Panggil Setempat | D-pdf |
| d-Entri Utama Nama Orang | |
| 500 Catatan Umum | Tidak dapat diakses di UIANA, karena: akan ditulis dalam bahasa Inggris untuk dipersiapkan terbit pada Jurnal Internasional yaitu Scientific Reports yang diprediksi akan dipublikasikan pada bulan Desember tahun 2022 |
| 337 Media Type | computer (rdamedia) |
| d-Entri Tambahan Nama Orang | |
| 526 Catatan Informasi Program Studi | Biologi |
| 100 Entri Utama Nama Orang | Fitrianingsih, author |
| 264a Kota Terbit | Depok |
| 300 Deskripsi Fisik | xii, 147 pages: illustration |
| 904a Pengisi Lembar Kerja | AdhityaN-Okt2022 |
| Akses Naskah Ringkas | |
| 856 Akses dan Lokasi Elektronik | |
| 502 Catatan Jenis Karya | Disertasi |
| 041 Kode Bahasa | eng |