Artikel Jurnal :: Kembali

Artikel Jurnal :: Kembali

Hydrocarbon degrading bacteria: isolation and identification

(Lembaga Penelitian Universitas Indonesia, 2007)

 Abstrak

There is little information how to identify hydrocarbon degrading bacteria for bioremediation of marine oil spills. We
have used gravel which contaminated oil mousse from Beach Simulator Tank, in Marine Biotechnology Institute,
Kamaishi, Japan, and grown on enrichment culture. Biostimulation with nutrients (N and P) was done to analyze
biodegradation of hydrocarbon compounds: Naphthalene, Phenanthrene, Trichlorodibenzofuran and Benzo[a]pyrene.
Community of bacteria from enrichment culture was determined by DGGE. Isolating and screening the bacteria on
inorganic medium contain hydrocarbon compounds and determination of bacteria by DAPI (number of cells) and CFU.
DNA was extracted from colonies of bacteria and sequence determination of the 16S rDNA was amplified by primers
U515f and U1492r. Twenty nine strains had been sequence and have similarity about 90-99% to their closest taxa by
homology Blast search and few of them have suspected as new species.

 Metadata

Jenis Koleksi : Artikel Jurnal
Subjek :
Penerbitan : [Place of publication not identified]: Lembaga Penelitian Universitas Indonesia, 2007
Sumber Pengatalogan :
ISSN :
Majalah/Jurnal : Makara: Sains
Volume : 11 (2) November 2007: 98-103
Tipe Konten :
Tipe Media :
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Akses Elektronik :
Institusi Pemilik :
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