UI - Skripsi Membership :: Back

UI - Skripsi Membership :: Back

Deteksi Rotavirus Grup A menggunakan Gen VP7 dan VP4 pada Feses Pasien Diare Pediatri di Makassar dengan Reverse Transcription-Nested Polymerase Chain Reaction

Gina Monita; Amarila Malik, supervisor; Putnam, Shannon D., supervisor (Fakultas Farmasi Universitas Indonesia, 2006)

 Abstract

Diare karena rotavirus adalah masalah kesehatan masyarakat di seluruh dunia. Rotavirus grup A yang menyerang manusia adalah penyebab terbesar dari penyakit gastroenteritis akut pada anak - anak baik di negara maju maupun negara berkembang. Rotavirus saat ini menjadi subjek penelitian dan ujicoba untuk pencarian vaksin yang efektif dan aman. Penelitian dilakukan untuk menentukan prevalensi rotavirus grup A di daerah Makassar selama bulan Oktober 2005 sampai Oktober 2006. Sampel feses dengan gejala diare dikumpulkan dari pasien pediatri sebanyak 326 sampel. Sampel kemudian diuji dengan metode ELISA dan menunjukkan 26,07% positif terinfeksi rotavirus grup A. Sampel positif tersebut kemudian dianalisis lebih lanjut dengan metode RT dan nested PCR menunjukkan bahwa prevalensi terbesar dari galur rotavirus grup A di daerah Makassar adalah serotipe G4G9P[8] sebanyak 36,55 (n = 31).

Rotavirus diarrhea is a public health problem throughout the world. Group A human rotaviruses are a major cause of acute gastroenteritis in young children in both developing and developed countries. Rotaviruses are at present the subject of intense vaccine research and trials worldwide to find an effective and safety vaccine. The study was conducted to determine the prevalence of group A rotavirus in Makassar on October 2005 until October 2006. Three hundred twenty six stool samples were collected from pediatric patient with diarrhea symptoms. The samples were tested by ELISA method and resulted as 26,07% positive of group A rotavirus. The ELISA positive samples were then analyzed by RT and nested PCR method, subsequently, and result showed that the major prevalence of group A rotavirus in Makassar that is 36,55% (n = 31) were G4G9P[8] serotype.

 Digital Files: 1

Shelf
 S33049-Gina Monita.pdf :: Download

LOGIN required

 Metadata

Collection Type : UI - Skripsi Membership
Call Number : S33049
Main entry-Personal name :
Additional entry-Personal name :
Study Program :
Subject :
Publishing : Depok: Fakultas Farmasi Universitas Indonesia, 2006
Cataloguing Source
Content Type
Media Type
Carrier Type
Physical Description xi, 64 hlm. : ill. ; 28 cm + lamp.
Concise Text
Holding Institution Universitas Indonesia
Location Perpustakaan UI, Lantai 3
  • Availability
  • Review
  • Cover
Call Number Barcode Number Availability
S33049 14-21-252412180 TERSEDIA
Review:
No review available for this collection: 20176707
Cover