Sterol dalam tumbuhan dan hewan merupakan salah s,tu bahan baku untuk produksi obat-obat kontrasepsi oral.Dalam rangka mencari umber sterol, telah didapat suatumetode identifikasi dan penetapan kadar sterol yang sedehana, yang dapat digunakan untuk minyak sawit kasar danberbagai fraksi limba.hnya yang terdiri dari sludge, tandan,sabut sisa pengempaan dan air limbah.Dari hasil identifikasi menggunakan pereaksi Lieberrnann-Burchard dan Kromatografj. Lapisan fipis, didapatbahwa niinyak sawit kasar dan semua fraksi limbahnya mengandungsterol.Penetapan kadar sterol tak jenuh pada ekstrak danfraksi tak tersabunkan dilakukan secara kolorimetri menggunakanpereaksi Liebermann-Burchard. Sampel-sampel yangmengandung zat-zat pengganggu dapat dimurnikan dengan Krmatografi Lapisan Tipis sebelum penetapan kadar0Tandan yang merupakan fraksi limbah terbesar mengandungsterol terbanyak.

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Sterols which occur in plants and animals can be

used as a raw material for production of oral contrasept

yes. To investigate possible resources of sterols, a simple

identification and assay method has been obtained,

which can be used for Crude Palm Oil and the various waste

fractions consisting of sludge, bunch, wasted mesocarp

and wasted water.

Identification by the Liebermann-Burchard reaction

and Thin Layer Chromatography show that Crude Palm Oil

and the various waste fractions concerned with its production

contain sterols.

The assay of total unsaturated sterols from the

extract of unsaponifiable matter was done by a colorime-.

tric method based on the Liebermann-Burchard reaction.

Samples containing a lot of disturbing substances can be

cleaned up by Thin Layer Chromatography prior to assay.

The bunch which is the majority of the waste has

the highest sterol content.