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Optimasi pereaksi Schryver dan penerapannya pada analisis Formaldehid dalam usus dan hati ayam spektrofotometri

Annisrakhma Swastiniar Kuswan; Herman Suryadi, supervisor; Nelly Dhevita Leswara, supervisor; Maryati Kurniadi, examiner; Berna Elya, examiner; Santi Purna Sari, examiner (Fakultas Farmasi Universitas Indonesia, 2011)
 Abstrak
[ABSTRAK
Pereaksi Schryver merupakan salah satu pereaksi yang biasa digunakan
untuk analisis kualitatif formaldehid. Pereaksi ini banyak digunakan karena
memiliki sensitivitas dan selektivitas yang baik terhadap formaldehid. Penelitian
ini bertujuan untuk melakukan optimasi pereaksi Schryver agar penggunaannya
optimal pada analisis kualitatif dan kuantitatif formaldehid. Optimasi dilakukan
dengan cara membuat variasi konsentrasi dari masing-masing komponen secara
bertahap. Komposisi yang optimum dipilih berdasarkan intensitas dan stabilitas
serapan yang diperoleh. Kemudian komposisi ini digunakan untuk mengidentifikasi
dan menentukan kadar formaldehid secara spektrofotometri pada sampel usus dan
hati ayam yang dijual di Pasar Minggu dan Pasar Kramat Jati, Jakarta, Indonesia.
Hasil validasi metode menunjukkan batas deteksi 0,0464 mg/L dan batas kuantitasi
0,1546 mg/L. Uji presisi dan akurasi metode menunjukkan hasil yang baik dengan
koefisien variasi 0,538%, persentase perolehan kembali formaldehid dalam sampel
usus ayam berkisar antara 98,64 ? 100,08% dan dalam sampel hati ayam 99,86 ?
104,34%. Identifikasi formaldehid terhadap 6 sampel usus ayam didapatkan hanya 1
sampel yang menunjukkan hasil yang positif dengan kadar 99,8481 μg/g. Sedangkan
identifikasi formaldehid dalam 6 sampel hati ayam menunjukkan hasil yang negatif.

ABSTRACT
Schryver?s Reagent is one of many reagent that often used for
formaldehyde qualitative analysis. For most purposes Schryver?s method using
phenylhydrazine hydrochloride and potassium ferricyanide is the method of
choice because this reagent have a good sensitivity and selectivity against
formaldehyde. In this research, the Schryver?s reagent will be optimized and so it
can be used optimally in qualitative and quantitative analysis of formaldehyde.
Optimization was done by varying the concentration of each component step by
step. The optimum composition was selected based on the absorption intensity
and stability obtained. Then the optimized composition will be used to identify
and determine the value of formaldehyde using spectrophotometry in chicken
intestine and liver samples that sold in Pasar Minggu and Pasar Kramat Jati,
Jakarta, Indonesia. The validation method showed that the detection limit was
0.0464 mg/L and the quantification limit 0.1546 mg/L. Precision and accuracy test
showed a good result that variation coeficient 0.538%, recovery test of
formaldehyde in chicken intestine sample is about between 98.64% and 100.08%,
and about between 99.86% and 104.34% in chicken liver. The formaldehyde
identification in chicken intestine sample showed from six samples have been
tested, only one sample that gave a positive result which contain 99.8481 μg/g.
Whereas the formaldehyde identification in chicken liver sample showed a
negative result., Schryver?s Reagent is one of many reagent that often used for
formaldehyde qualitative analysis. For most purposes Schryver?s method using
phenylhydrazine hydrochloride and potassium ferricyanide is the method of
choice because this reagent have a good sensitivity and selectivity against
formaldehyde. In this research, the Schryver?s reagent will be optimized and so it
can be used optimally in qualitative and quantitative analysis of formaldehyde.
Optimization was done by varying the concentration of each component step by
step. The optimum composition was selected based on the absorption intensity
and stability obtained. Then the optimized composition will be used to identify
and determine the value of formaldehyde using spectrophotometry in chicken
intestine and liver samples that sold in Pasar Minggu and Pasar Kramat Jati,
Jakarta, Indonesia. The validation method showed that the detection limit was
0.0464 mg/L and the quantification limit 0.1546 mg/L. Precision and accuracy test
showed a good result that variation coeficient 0.538%, recovery test of
formaldehyde in chicken intestine sample is about between 98.64% and 100.08%,
and about between 99.86% and 104.34% in chicken liver. The formaldehyde
identification in chicken intestine sample showed from six samples have been
tested, only one sample that gave a positive result which contain 99.8481 μg/g.
Whereas the formaldehyde identification in chicken liver sample showed a
negative result.]
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 Metadata
No. Panggil : S1078
Nama orang :
Nama orang tambahan :
Nama badan tambahan :
Subjek :
Penerbitan : [Place of publication not identified]: Fakultas Farmasi Universitas Indonesia, 2011
Program Studi :
Kode Bahasa : ind
Sumber Pengatalogan : LibUI ind rda
Tipe Konten : text
Tipe Media : unmediated ; computer
Tipe Carrier : volume ; online resources
Deskripsi Fisik : xv, 91 pages : illustration ; 30 cm + appendix
Catatan Bibliografi : page 48
Lembaga Pemilik : Universitas Indonesia
Lokasi : Perpustakaan UI, Lantai 3
  • Ketersediaan
  • Ulasan
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No. Panggil No. Barkod Ketersediaan
S1078 TERSEDIA
Ulasan:
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