Clostridium difficile is the most important cause of antibiotic associated diarrhea, and pseudomembranous colitis, a severe infection of the colon. Strain Clostridium difficile produce two potent toxin, toxin A (enterotoxin) and toxin B (cytotoxin). These two toxins are both responsible for the diarrhea and inflammation seen in patients treated due to infection, especially the broad spectrum antibiotics. Direct detection of Clostridium difficult cytotoxin front faecal specimen using mammalion tissue culture lines is considered the standard diagnostics test of Clostridium difficult infection. This test is very sensitive but requires a minimum two days to complete. In order to improve the threshold of diagnosis and treatment, a number of enzyme immunoassay methods have been used, with a reported sensitivity to either toxin A or toxin B.