Mannan is an oligo-saccharide that was used for human health as a prebiotic food and is produced
from hydrolysis of palm kernel cake, coconut seed copra and coke waste. Domestically, palm kernel
cake and copra utilized for low efficiency metabolized animal feed A t this moment, via glico-science and
technology route, these farming waste using mannanase could be degraded to produce functional oligo
saccharide such as mannose and manno-oligosaccharide. Around 20-40% of palm kernel cake fibrous
from CPO waste consist beta-mannan. In the beginning, palm kernel cake fibrous qualitative and
quantitative analysis and mannan preparation was done for the next enzymatic processing (fermentation
step). Optimum condition of palm kernel cake residue hydrolysis was 110°C used heating process till 1_5
hour and 2% mass acid catalyst of 150 g/L, palm kernel cake residue solution. Here, hydrolysis 150 g/L
substrate solution at 90" C used heating process till 1.5 hour produced 19.1% mass mannan. Hydrolisis
using Streptomyces and Saccharopolyspora (strains from B TCC) resulted that both isolates have a
potential to produce mannanase and this raw enzyme qualitativlyt capable to hydrolysis palm kernel cake
for producing oligo-saccharides. Both isolates produced mannanase which higher specific activity after
24 hour inoculation of palm kernel cake residue solution.
