ABSTRAK
Xanthorrhizol yang di isolasi dari Temulawak dapat mempertahankan pH modelbiofilm in vitro selama 4 jam. Diketahui KBM ekstrak Temulawak terhadap S.mutans 25% Tujuan : Menganalisis efek ekstrak Temulawak 25% terhadapdemineralisasi email yang terpapar biofilm S.mutans Metode : Model biofilmdiperoleh dengan mengkultur S.mutans yang sudah ditanam dalam TYS brothselama 24 jam pada 6 well ?plate yang telah dilapisi pelikel. Ekstrak Temulawakdipaparkan pada model biofilm pada berbagai durasi antara 1-48 jam. PengukuranpH menggunakan pH universal indicator. Model biofilm juga ditumbuhkan padapermukaan sample gigi. Pemaparan Ekstrak Temulawak dilakukan pada jam ke16-20. Uji kekerasan mikro menggunakan indenter Knoop sebelum dan sesudahperlakuan. Hasil : Sampai dengan jam ke 4, pH model biofilm yang terpaparEkstrak Temulawak 25% tidak mengalami penurunan pH. Tidak terlihat efekEkstrak Temulawak terhadap kekerasan permukaan email.Kesimpulan : EkstrakTemulawak 25% mampu menghambat penurunan pH biofilm, tetapi tidakberpengaruh terhadap demineralisasi email.

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ABSTRACT
Xanthorrhizol isolated from Java turmeric is able to maintain the pH of biofilmmodel in vitro for 4 hours. It was known that MBC of Java turmeric extract was25%. Purpose: To analyse the effect of 25% Java turmeric extract on emaildemineralization exposed to S. mutans biofilm. Methods: Biofilm model wasobtained by culturizing S. mutans which was cultured on TYS Broth during 24hours on 6 well- plates which was layered by pellicle. Java turmeric Extract wasadded to biofilm model at various duration between 1-48 hours. pH measurementusing pH universal indicator. Biofilm model was also cultured at tooth samplesurface. Java turmeric extract was added at 16-20 hours. Micro hardness test wasconducted using Knoop indenter before and after the intervention. Result : After4 hours, the pH of biofilm model which was exposed to Java turmeric 25% wasnot decreasing. No difference was found on the enamel micro hardness betweenexperiment and control groups. Conclusion : Java turmeric 25% is able to preventreduction of biofilm pH, but does not have effect on enamel demineralization.