Kobalt klorida dapat digunakan sebagai senyawa yang dapat menimbulkan kondisi mimikri hipoksia tanpa kadar rendah oksigen di dalam tubuh, dan menstabilkan hypoxia inducible factor-1α. Kami memutuskan untuk mengobservasi apakah terdapat regulasi ekspresi renin oleh HIF-1α. Dengan demikian kami menyelenggarakan beberapa penelitian untuk memastikan kemungkinan dan memulai dengan penelitian induksi tikus secara intraperitoneal kobalt klorida (CoCl2) untuk membangkitkan kondisi mimikri hipoksia dan mendapatkan konsentrasi dan pola ekspresi HIF-1α dan mRNA.Metode: Dua puluh empat ekor tikus dibagi menjadi 4 grup: kontrol, 2, 8, dan 24 jam inkubasi pasca injeksi intraperitoneal 30 mg/kg berat badan CoCl2. Setelah tikus dikorbankan, organ ginjal digunakan untuk pemeriksaan parameter berat ginjal, kadar RNA, kadar protein HIF-1α (ELISA) dan mRNA renin (RT-PCR). Hasil: Hasil menunjukkan bahwa terdapat perbedaan rasio berat ginjal/berat badan tikus, namun secara statistik tidak bermakna (p > 0,05). Secara statistik tidak terdapat perbedaan bermakna kadar protein HIF-1α antar kelompok (p > 0,05). Ekspresi relatif mRNA renin meningkat tajam (30 x kontrol), mulai pada 8 jam inkubasi pasca induksi intraperitoneal CoCl2 dan terus meningkat sampai inkubasi 24 jam (2465 x kontrol). Korelasi antara protein HIF-1α dan ekpresi relatif mRNA renin menggunakan analisis Pearson menunjukkan positif kuat (R = 0,91) (p = 0,09). Kesimpulan: Terdapat kemungkinan yang besar bahwa gen renin diregulasi oleh HIF-1α.

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Abstract
Background: Cobalt chloride can be used as an agent to stabilize hypoxia inducible factor-1α (HIF-1α) and to imitate hypoxia without low levels of oxygen inside the body. We intended to investigate if there was any regulation of renin expression by HIF-1α. Therefore, we conducted several studies to clarify this possibility starting with the induction of hypoxic mimicry in rats by intra-peritoneal (IP) injection of cobalt chloride (CoCl2) to obtain the levels and pattern of HIF-1α and renin mRNA and protein expression.Methods: Twenty-four rats were randomly divided into four groups, control group and incubation groups 2, 8, and 24 hours after intra-peritoneal injection of 30 mg CoCl2 per kg BW. After the rats were sacrificed, kidneys were excised, weighed and kidney weight compared to BW. Tissue parameters were measured such as RNA concentration, HIF-1α protein by ELISA, and renin mRNA by RT-PCR.Results: Differences between the groups in the ratios of kidney weight to BW and in the concentrations of HIF-1α protein were statistically not significant (p > 0.05). Relative expression of renin mRNA increased markedly starting 8 hours after CoCl2 IP injection (30 times over controls) and further rising until 24 hours (2465 times over controls). Correlation between HIF-1α and renin mRNA by Pearson analysis was strongly positive, but not significant (R = 0.91; p = 0.09).Conclusion: Renin gene regulation in renal hypoxic mimicry strongly correlates with HIF-1α