The number of malaria cases in the world is 350- 500 million and more than one minion deaths occur every year, particularly intropical area and Africa. The prevalence of malaria in Indonesia is 2.85%. There are 49.6% Indonesian people which is risky to get infected with malaria because of living in an endemic malaria area. The prevalence of malaria in South Sumatera is 1.01 % and OKU District is the most endemic malaria area in South Sumatera with number of AMI (Annual Malaria Incidence) in 2008. of23.4%.In genera1t the objective of study was to assess the relation between the use of anti-mosquito lotion and incidence of malaria at Pengandonan, and the specific objectives are :1. To assess the relation between the use of anti mosquito lotion and incidence of malaria 2. To assess porentiaJ impact of the use of anti­ mosquito lotion and incidence of malaria at Pengandorum. OKU District.The study is an observational epidemiology in case control study design. The target population of the study was people of OKU District, while the actual population was people who live in Pengandonan and participated in MBS. The sample population was people who participated in MBS and recruited as sample study.The use of anti-mosquito lotion has significant influence with malaria incidence ofOR=0,312 p=O,OOO (95% CI 0,19-0,056), which means people who use anti mosquito lotion is only 0.312 times more risky to get infected with malaria compare to people who don't. The house construction has significant relation with malaria incidence of0R=7,88 p=O,OOO (95% Cl4,74-13,12).The knowledge of respondent about malaria has significant relation with malaria incidence of OR=0,49 dan p=O,OOJ (95% CI 0,31-0,78). Variable of "have acted around house» (IF 0.519) and variable of "diSstance of mosquito bedding"(p=O.l35) has no significant relation with malaria incidence. people who use anti-mosquito lotion is only 0.312 times more risky to get infected with malaria compare to people who don't. Public health intervention such as use of mosquito lotion will provide potential impact in decreasing malaria incidence in community of 53%.Suggestions:1. Efforts need to use anti-mosquito lotion that can be affordable by the community.considering the use of anti-mosquito lotion to prevent mosquito bites when outside the home is very effective and efficient.2. Increased knowledge of community with intensive counselling efforts so the knowledge of community about the disease malaria can be better, so they can be aware of their own willingness to seek a variety of activities that are preventive to mosquito bites.