ABSTRAKThe use of natural products as medicines has been known since
long time ago. Until nowadays plants are still being the primary source for
the treatment of a wide variety of diseases by the great civilizations in the
world. Indonesia has a high species richness of Garcinia (Garcinia spp.)
and it is as an important basic materials for breeding of bioactive chemical
compounds for medicines. One of species from Garcinia genus which
grows in Papua (Indonesia) is Garcinia maluensis Lauterb that belong to
Guttiferae (Clusiaceae) family.
This research was intended to isolate and determine the structure
of chemical compounds from the stem bark of G. Maluensis Lauterb, as
well as to conduct bioactivity test comprising a preliminary test to brine
shrimp lethality test (BSLT) and antioxidant test by using DPPH (1,1-
difenil-2-pikrilhidrazil) method from the isolated compunds. The isolation
was conducted by technique of chromatographyc combinations and
structure of isolated compounds were established by spectroscopy data:
mass spectrometry (MS), IR, UV, 1H-NMR, 13C-NMR and 2-D NMR,
included HMQC and HMBC.
The research founded mixture two compounds of
polyisoprenylbenzophenones (GML-1, GML-2 and GML-4). One of
compound which has molecular formula C38H50O6 was predicted as
camboginol and the other compound which molecular formula C38H48O6
was predicted as (Z)-3-(3,4-dihidroxibenzoil) hidroxi-1-(3-methilbut-2aenyl)-
5-((E)-2-(3-methilbut-2-enyl)hept-3-enyl)-7-(3-methilbutenylidene) bisiclo
[3,3,1]non-3-ene2,9 dione (GML-2 dan GML-4 = cayubenzofenon). GML-3
compound has molecular formula C38H50O6 was predicted as epicambogin
and GML-5 which molecular formula C29H48O was predicted as stigmasterol_
The result of the preliminary brine shrimp lethality test showed that
compounds GML-1, GML-2, GML-3 and GML-4 were toxic with each LC50
was 2,72; 1,64; 7,34 dan 1,68 ug/mL. The result of antioxidant test to
DPPH on the GML-1, GML-2, GML-3 and GML-4 showed antioxidant
DPPH radical scavenging with lC50 13,92; 12,59; 23,27 dan 13,95 ug/mL.
GML-5 did not show toxicity and antioxidant activity significantly.
Toxicity and ability to inhibite free radical reaction of DPPH (1,1-
difenil-2-pikrilhidrazil) of GML-1, GML-2, GML-3 and GML-4 were
predicted because of hidroxy groups existention on that compounds which
give the polarity and have function as radical scavenging and so that
inhibited the free radical reaction goes on.
For getting pure compound from mixture of GML-1, GML-2 and
GML-4 need the next tecknical purification. The further isolation of the
others fraction was needed for founding the others compound which have
more interesting bioactivities. Also need to do the anticancer and
antibacterial test or others bioactivites test from there compounds.
