ABSTRAK
TujuanUntuk mengetahui reliabilitas pemeriksaan hematoksilin eosin (HE) dan panelimunohistokimia (IHK) dalam membedakan pseudotumor dan limfoma malignadi mata dengan menilai intraobserver agreementMetodeDeskriptif analitik. Peneliti yang tersamar meninjau ulang sediaan HE dankombinasi HE dengan panel IHK yang terdiri dari CD20, CD3, CD43, Ki67, Bcl-2 . Evaluasi HE dilakukan 3 kali, sedangkan evaluasi IHK dilakukan 2 kalidengan interval 2 minggu. Intraobserver agreement dihitung dengan nilai kappa.Ekspresi masing masing penanda akan dideskripsikan secara kuantitatif.Predominansi limfosit dihitung dengan rasio CD20 terhadap CD3 dan CD43.HasilNilai kappa evaluasi HE adalah moderate (! = 0.59-0.80), sedangkan kombinasiHE dan panel imunohistokimia adalah excellent (! = 0.93). Median (kisaran)CD20 lebih tinggi pada limfoma [97.41 (80-100)] dibandingkan pseudotumor[80.79 (1-94.49)]. Median (kisaran) CD3 lebih tinggi pada pseudotumor [48.84(2.32-64.27)] dibandingkan limfoma [36.19 (0.01-82.13)]. Median (kisaran) CD43 relatif sama pada limfoma [73.81 (9.28-100)] dan pseudotumor [72.36 (46.55-100)]. Median (kisaran) Bcl2 relatif sama pada limfoma [85.22(0.37-99.54)] danpseudotumor [78.95 (64.24-91.73)]. Median (kisaran) Ki67 relatif sama rendahpada limfoma [11.13 (1.89-93.54)] dan pseudotumor [6.59 (0.05-49.60)]. Medianrasio CD20:CD3 limfoma [2.75 (1.12-8766)] lebih tinggi dibandingkanpseudotumor [1.60 (0.43-2.74)].

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ABSTRACT
ObjectiveTo investigate the reliability of haematoxylin eosin (HE) staining andimmunohistochemistry (IHC) panel in differentiating ocular adnexal lymphomaand pseudotumor by measuring intra observer agreement.MethodsA descriptive analytical study. Haematoxylin eosin staining and IHC panels(CD20, CD3, CD43, Ki67, Bcl-2) of 32 cases (15 lymphoma and 17pseudotumor) were reviewed by a pathologist whose blinded to its previousdiagnosis. The review was performed twice in two weeks apart. The intra observeragreement was analyzed using ! statistics. IHC markers were analyzedquantitatively. Lymphocytes predominance was indicated by CD20 ratio to CD3or CD43.ResultsThe ! statistics of HE stainings evaluation was moderate (! = 0.59-0.80), whilethat of HE and IHC panel was excellent (! = 0.93). Median (range) of CD20 washigher in lymphoma [97.41 (80-100)] compared to that of pseudotumor [80.79 (1-94.49)]. Median (range) of CD3 was higher in pseudotumor [48.84 (2.32-64.27)]compared to that of lymphoma [36.19 (0.01-82.13)]. Median (range) of CD 43was comparable in lymphoma [73.81 (9.28-100)] and that of pseudotumor [72.36(46.55-100)]. Median (range) of Bcl2 was comparable in lymphoma [85.22(0.37-99.54)] and that of pseudotumor [78.95 (64.24-91.73)]. Median (range) of Ki67was comparably low both in lymphoma [11.13 (1.89-93.54)] and pseudotumor[6.59 (0.05-49.60)]. Median (range) of CD20:CD3 ratio of lymphoma [2.75 (1.12-8766)] was higher than that of pseudotumor [1.60 (0.43-2.74)].