Daun benalu mangga telah diketahui mampu memiliki aktivitas antioksidan, namunbelum diketahui potensinya sebagai penghambat tirosinase. Aktivitas antioksidanberpotensi sebagai penghambat tirosinase yang bergunau ntukmenghambatterbentuknya melanin di dalam kulit. Penelitian ini bertujuan untuk memperolehaktivitas penghambatan tirosinase dan manfaat dari sediaan emulgel ekstrak etanoldaun benalu mangga. Metode yang digunakan adalah DPPH (1,1difenildipikrilhidrazil) untuk mengetahui aktivitas antioksidan dan Dopakrom untukmengetahui penghambatan terhadap enzim tirosinase.Parameter adanya aktivitas yangdimiliki ekstrak ditunjukan oleh nilai IC50 dan persentase inhibisi. Ekstrak etanoldaun benalu mangga dibuat sediaan emulgel dengan konsentrasi 0,5%. Uji stabilitasfisik sediaan emulgel dilakukan selama 12 minggu dan uji keamanan kepadasukarelawan menggunakan metode uji tempel. Uji manfaat dilakukan selama 28 haripada daerah lengan bawah dengan parameter indeks melanin kulit. Hasil ekstraksimenunjukkan ekstrak etanol daun benalu mangga memiliki rendemen 59,89%.Pengujian terhadap aktivitas antioksidan dan inhibitor tirosinase masing-masingmemiliki nilai IC50sebesar 31,41 µg/mL dan 722,73 µg/mL. Sediaan emulgel ekstraketanol daun benalu mangga belum menunjukkan kestabilan selama 12 minggu,namun hasil uji keamanan tidak menimbulkan iritasi sehingga aman digunakan secaratopikal. Hasil uji manfaat menunjukkan sediaan emulgel ekstrak daun benalu manggabelum mampu mencerahkan kulitsecara signifikan dalam waktu 28 hari.

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Mango mistletoe leaves ethanol extract has been known have antioxidant activity, but

it has not known as a potential inhibitor of tyrosinase. The antioxidant activity can be

potentially useful as tyrosinase inhibitors to inhibit the melanin in the skin. The aims

of the study is to obtain tyrosinase inhibitor activity and to get the benefits of mango

mistletoe leaves ethanol extract emulgel. The method used was DPPH

(1,1diphenyldipikrilhidrazil) to determine the antioxidant activity and Dopakrom to

determine the inhibition of the enzyme tyrosinase. The parameters of the activity

shown by the extracts possessed IC50values and the percentage of inhibition. The

concentration of emulgel from mango mistletoe leaves ethanol extract was 0.5%.

Physical stability test of emulgel performed for 12 weeks and safety test to volunteers

was using a patch test method. Efficacy test conducted for 28 days in the area of the

forearm with skin melanin index as the parameter. Extraction results showed mango

mistletoe leaves ethanol extract has a yield about 59.89%. The test result of

antioxidant activity and tyrosinase inhibitors based on IC50 values are 31.41 µg/mL

and 722.73 µg/mL. Emulgel of mango mistletoe leaves ethanol extracthas yet stable

for 12 weeks, however the results of safety test showedthat there was no irritation on

the skin so it was safe to use topically. The efficacy test results demonstrated the

benefits of emulgel of mango mistletoe leaves ethanol extract has not been able to

lighten skin significantly within 28 days.