[Penyakit infeksi merupakan salah satu masalah utama di negara berkembang saat ini.
Keadaan ini memicu peningkatan frekuensi pemakaian anti mikroba sehingga resiko
panresisten semakin besar. Tujuan penelitian ini adalah untuk melihat gambaran MIC
mikroba panresisten terhadap anti mikroba yang paling sering digunakan di lingkungan
RSUPNCM. Mikroba yang diuji merupakan mikroba panresisten yang terbanyak berdasarkan
peta kuman yang disusun oleh Loho dkk pada bulan juli - desember 2012.
Penelitian potong lintang ini terdiri dari 90 mikroba panresisten yaitu Acinetobacter
spp, Pseudomonas spp dan Klebsiella spp. Data karakteristik sampel pemeriksaan dicatat dan
dilakukan uji resistensi. Uji resistensi mengunakan metode difusi cakram dan metode MIC
strip test kemudian dicari 'interpretative error' kedua metode.
Pada ketiga mikroba yang panresisten dengan metode difusi cakram diketahui bahwa
masih terdapat nilai intermediate dan sensitif dengan metode MIC strip test. Pada
Acinetobacter spp nilai sensitif paling tinggi didapat pada anti mikroba CS sebesar 55 isolat
(100%) dan diikuti dengan anti mikroba AK sebanyak 7 isolat (12,7%) dan oleh CAS 1 isolat
(1.8%). Nilai intermediate tertinggi terdapa pada AK yaitu 6 isolat (10,9%) yang diikuti oleh
CAS 1 isolat (1.8%). Pada Pseudomonas spp yang diperoleh nilai sensitif terbesar pada anti
mikroba CS 17 isolat (89.5%) diikuti dengan AK yaitu sebesar 3 isolat (15.7%), Nilai
intermediate tertinggi terdapat pada AK yaitu 6 isolat (31,5%) yang diikuti oleh CAS 4 isolat
(21.1%) dan CS 2 isolat (10.5%). Klebsiella spp dengan jumlah 16 isolat didapatkan nilai
sensitif tertinggi pada CS 16 isolat (100%) diikuti DOR dengan 2 isolat (12.5%) dan
kemudian oleh AK dan CAS dengan masing masing 1 isolat (6.25%). Nilai intermediate
terdapat pada DOR dan AK dengan masing masing 1 isolat (6.25%).
Hasil nilai ''interpretative error'' menunjukan tidak ditemukan VME. Nilai MJE dan
MNE Acinetobacter spp yang melebihi kriteria FDA adalah AK yaitu 12.7% dan 10.9%.
Pada Pseudomonas spp nilai MJE yang melebihi kriteria FDA adalah AK, CAS dan DOR
yaitu 15.7%, 10,5% dan 5,2%. Nilai MNE yang melebihi kriteria FDA adalah AK, CAS dan
CS yaitu 31,5%, 21,1% dan 10,5%. Pada Klebsiella spp nilai MJE pada DOR, AK dan CAS
yaitu 12.5%, 6.25% dan 6.25%. Nilai MNE pada AK dan DOR yaitu 6.25% dan 6.25%.
Kami menyimpulkan bahwa untuk mikroba yang panresisten dengan metode difusi
cakram dapat digunakan MIC test.;Infectious diseases are one of the major problems in developing countries today. This
condition triggers an increase in frequency of anti-microbial used that leads increase
panresistance. The purpose of this study was to descriptive of the MIC microbes resistant to
anti-microbial is most often used in RSUPNCM. Microbes tested is the most resistant
microbes germs based map compiled by Loho et al in July - December 2012.
This cross-sectional who study consisted of 90 microbes resistant ie. Acinetobacter
spp, Pseudomonas spp and Klebsiella spp. The characteristics of the data sample are recorded
and performed resistance test. Resistance test its done using the disc diffusion and MIC test
strip method and then analized 'interpretative error' for both methods.
On the third microbial panresisten with disc diffusion method is known that there are
intermediate values and sensitive with MIC method. In Acinetobacter spp sensitive highest
value whose obtained on the anti- microbial CS by 55 isolates (100 %) followed by antimicrobial
and AK were 7 isolates (12.7 %) and by CAS 1 isolates ( 1.8 % ). The highest
intermediate value ie there exist at AK 6 isolates (10.9 %) , followed by CAS 1 isolates (1.8
%). In Pseudomonas spp were most sensitive to the values whose obtained anti- microbial CS
17 isolates (89.5 %) followed by the AK is equal to 3 isolates (15.7 %) , the highest
intermediate value that is contained in AK 6 isolates ( 31.5 % ) , followed by CAS 4 isolates
(21.1 %) and CS 2 isolates (10.5 %) . Klebsiella spp isolates whose obtained with the number
16, the highest score on the CS 16 sensitive isolates (100 %) followed DOR with 2 isolates
(12.5 %) and then by AK and CAS with each 1 isolates (6,25 %). Intermediate values
contained in DOR and AK with each 1 isolates ( 6,25 %) .
Results of 'interpretative error'' is not found in Very major error. Minor error and
Major error Acinetobacter spp that exceeds the FDA criteria are AK is 12.7% and 10.9%. In
Pseudomonas spp Major error value that exceeds the FDA criteria are AK, CAS and DOR
with 5.7%, 10.5% and 5.2%. The Minor error value that exceeds the FDA criteria are AK,
CAS and CS are 31.5%, 21.1% and 10.5%. In Klebsiella spp, Major error on DOR, AK and
CAS with 12.5%, 6:25% and 6:25%. The Minor error value on AK and DOR is 6:25% and
6:25%.
We conclude that MIC test can be used for microbial panresisten with the disc
diffusion., Infectious diseases are one of the major problems in developing countries today. This
condition triggers an increase in frequency of anti-microbial used that leads increase
panresistance. The purpose of this study was to descriptive of the MIC microbes resistant to
anti-microbial is most often used in RSUPNCM. Microbes tested is the most resistant
microbes germs based map compiled by Loho et al in July - December 2012.
This cross-sectional who study consisted of 90 microbes resistant ie. Acinetobacter
spp, Pseudomonas spp and Klebsiella spp. The characteristics of the data sample are recorded
and performed resistance test. Resistance test its done using the disc diffusion and MIC test
strip method and then analized 'interpretative error' for both methods.
On the third microbial panresisten with disc diffusion method is known that there are
intermediate values and sensitive with MIC method. In Acinetobacter spp sensitive highest
value whose obtained on the anti- microbial CS by 55 isolates (100 %) followed by antimicrobial
and AK were 7 isolates (12.7 %) and by CAS 1 isolates ( 1.8 % ). The highest
intermediate value ie there exist at AK 6 isolates (10.9 %) , followed by CAS 1 isolates (1.8
%). In Pseudomonas spp were most sensitive to the values whose obtained anti- microbial CS
17 isolates (89.5 %) followed by the AK is equal to 3 isolates (15.7 %) , the highest
intermediate value that is contained in AK 6 isolates ( 31.5 % ) , followed by CAS 4 isolates
(21.1 %) and CS 2 isolates (10.5 %) . Klebsiella spp isolates whose obtained with the number
16, the highest score on the CS 16 sensitive isolates (100 %) followed DOR with 2 isolates
(12.5 %) and then by AK and CAS with each 1 isolates (6,25 %). Intermediate values
contained in DOR and AK with each 1 isolates ( 6,25 %) .
Results of 'interpretative error'' is not found in Very major error. Minor error and
Major error Acinetobacter spp that exceeds the FDA criteria are AK is 12.7% and 10.9%. In
Pseudomonas spp Major error value that exceeds the FDA criteria are AK, CAS and DOR
with 5.7%, 10.5% and 5.2%. The Minor error value that exceeds the FDA criteria are AK,
CAS and CS are 31.5%, 21.1% and 10.5%. In Klebsiella spp, Major error on DOR, AK and
CAS with 12.5%, 6:25% and 6:25%. The Minor error value on AK and DOR is 6:25% and
6:25%.
We conclude that MIC test can be used for microbial panresisten with the disc
diffusion.]
SP-Riana.pdf :: Unduh