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Karakterisasi penghambatan fusarium oxysporum schlecht f. sp. cubense foc oleh dua isolat aktinomisetes secara in vitro = Characterization of in vitro inhibition activity from two actinomycetes isolates toward fusarium oxysporum schlecht f sp cubense

Dwi Apriyanto; Sarjiyo Antonius, supervisor; Sitaresmi Ismangil, examiner; Iman Santoso, examiner; Ratna Yuniati, examiner (Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2014)

 Abstract

Penyakit Panama pada tanaman pisang disebabkan oleh kapang patogen Fusarium oxysporum f. sp. cubense. Telah dilakukan penelitian untuk karakterisasi penghambatan Aktinomisetes terhadap Fusarium oxysporum f. sp. cubense secara in vitro menggunakan sel hidup dan filtrat kultur bebas sel. Isolat Aktinomisetes LAI-I dan L31 diketahui menghasilkan enzim kitinase, protease, dan antibiotik yang dapat menghambat pertumbuhan, menyebabkan perubahan morfologi hifa berupa penebalan pada ujung-ujung hifa, serta menghambat germinasi spora Fusarium oxysporum f. sp. cubense. Perhitungan statistik menunjukkan adanya perbedaan yang nyata antara kelompok kontrol, perlakuan LAI-I, dan perlakuan L31. Hasil tersebut memperlihatkan adanya kemampuan isolat LAI-I dan L31 menghambat Fusarium oxysporum f. sp. cubense.

The Panama disease in banana plants is caused by the pathogenic fungi Fusarium oxysporum f. sp. cubense. Research has been conducted to characterize the inhibition mechanism of Actinomycetes towards Fusarium oxysporum f. sp. cubense in vitro by using living cell and cell-free culture filtrate. Actinomycetes isolates LAI-I and L31 produce chitinase enzyme, protease enzyme, and secondary metabolites that can inhibit the growth, lead morphological changes of hyphae as swollen at the end of the hypha, and inhibit spore germination of Fusarium oxysporum f. sp. cubense. The statistic reveals the significant differences between control, LAI-I treatment, and L31 treatment. The result shows the ability of isolate LAI-I and L31 to inhibit Fusarium oxysporum f. sp. cubense.

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 Metadata

Collection Type : UI - Skripsi Membership
Call Number : S53974
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Publishing : Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2014
Cataloguing Source LibUI ind rda
Content Type text
Media Type unmediated ; computer
Carrier Type volume ; online resource
Physical Description xiv, 55 pages : illustration ; 28 cm + appendix
Concise Text
Holding Institution Universitas Indonesia
Location Perpustakaan UI, Lantai 3
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Call Number Barcode Number Availability
S53974 14-25-98898262 TERSEDIA
Review:
No review available for this collection: 20367859
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