[Karies merupakan penyakit infeksi pada gigi yang paling sering menjadi masalah
bagi kesehatan mulut. Prevalensi karies di Indonesia tergolong tinggi. Penyebab
utama karies adalah bakteri Streptococcus mutans. Beberapa penelitian menunjukkan
daun binahong memiliki zat antibakteri. Penelitian ini bertujuan untuk membuktikan
efek antibakteri infusum daun binahong terhadap Streptococcus mutans secara in
vitro. Konsentrasi infusum yang diuji 100%, 50%, 25%, dan 10%. Uji dilusi
dilakukan untuk mendapatkan nilai KHM dan KBM, menggunakan media BHI broth
dan TYS20B. Uji difusi dilakukan untuk memperoleh nilai zona hambatan,
menggunakan media BHA dan BHA-darah. Dari uji dilusi, didapatkan nilai KHM
50% dan KBM diatas 50%. Dari uji difusi pada media BHA, didapatkan nilai zona
hambatan: 0,75 mm (10%), 0,625 mm (25%), 1,125 mm (50%), 0,75 mm (100%).
Dari uji difusi pada media BHA-darah, didapatkan nilai zona hambatan: 0,5 mm
(10%), 0,875 mm (25%), 1,125 mm (50%), 0,625 mm (100%). Bakteri S. mutans
sensitif terhadap infusum daun binahong., Dental caries is the most common oral infectious disease in humans with relatively
high prevalence in Indonesia. Streptococcus mutans is the main causative agent of
caries. Previous researches had identified several antibacterial ingredients in
binahong leaves. This study is aimed to prove that binahong leaves infusion is
effective as an antibacterial agent against Streptococcus mutans in vitro. The
concentration of infusion that were used in this test were 100%, 50%, 25, and 10%.
To determine minimum inhibitory concentration (MIC) and minimum bactericidal
concentration (MBC), dilution test was performed on BHI broth and TYS20B
mediums. As to measure zone of inhibition, diffusion test was performed on BHA
and BHA-blood mediums. The result showed that the MIC was 50% and the MBC
was above 50%. From the diffusion test on BHA medium, the scores of inhibitory
zone are 0,75 mm (10%), 0,625 mm (25%), 1,125 mm (50%), 0,75 mm (100%).
While on BHA-blood medium, the scores are 0,5 mm (10%), 0,875 mm (25%), 1,125
mm (50%), 0,625 mm (100%). It is concluded that S. mutans is sensitive to binahong
leaves infusion.]
S-veronica_lakshmi_maharani_putri.pdf :: Unduh