[Belum ada prosedur baku sentrifugasi untuk pemisahanprotein. Dilaporkan bahwa sentrifugasi 10.000 g dapat memisahkan proteinsaliva ≥30 kDa. Tujuan: Mengetahui pengaruh kecepatan sentrifugasi 7.000g, 8.000 g, dan 9.000 g terhadap frekuensi kemunculan dan profil proteinsaliva ≥30 kDa. Metode: Profil protein supernatan saliva hasil sentrifugasidiuji dengan SDS-PAGE Hasil: Frekuensi kemunculan protein ≥30 kDamengalami penurunan sesuai peningkatan kecepatan sentrifugasi. Terdapatperbedaan profil protein antara hasil sentrifugasi 7.000 g, 8.000 g, dan 9.000g. Kesimpulan: Kecepatan sentrifugasi 7.000 g, 8.000 g, dan 9.000 gberpengaruh terhadap frekuensi kemunculan dan profil protein ≥30 kDa., There are no established standard operational procedure ofcentrifugation for protein separation. Centrifugation at 10.000 g separatessalivary protein ≥30 kDa. Objective: To determine the effect ofcentrifugation at 7.000 g, 8.000 g, and 9.000 g on the frequency of salivaryprotein emergence and protein profile ≥30 kDa. Method: Salivarysupernatant were analyzed with SDS-PAGE. Results: Increasedcentrifugation speed resulted in decreased frequency of protein ≥30kDa.There are differences in the protein profiles between the results of eachcentrifugation. Conclusion: Centrifugation at 7.000 g, 8.000 g,and 9.000 ginfluence frequency of salivary protein emergence and protein profiles ≥30kDa.]