[ABSTRAK
Telah dilakukan penelitian untuk mendeteksi Shiga Toxin-producing Escherichiacoli (STEC) pada daging Perna viridis (kerang hijau) dan Anadara granosa(kerang darah) yang berasal dari pasar tradisional, swalayan, dan tempat budidayakerang di Cilincing serta Muara Kamal. Rangkaian uji yang digunakan adalahMultiple Tube Fermentation (MTF), hemolisis dan teknik molekular untukmendeteksi gen spesifik shiga toxin (stx1, stx2), intimin (eaeA) dan hemolisin(hlyA). Hasil uji MTF menunjukkan bahwa kandungan bakteri E. coli dalam dagingkerang melebihi ambang batas keamanan pangan SNI No. 01-2729-3-2006 (> 200MPN/100 g). Hasil uji hemolisis menunjukan bahwa 59,4 % bakteri E. coli yangdiisolasi dari daging kerang mampu melisiskan sel darah merah. Gen penyandiSTEC tidak ditemukan pada sampel daging kerang

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ABSTRACT
A study was carried out to detect Shiga Toxin-producing Escherichia coli (STEC)in Perna viridis (green mussel) and Anadara granosa (blood cockle) fleshs.Shellfish fleshs were obtained from traditional markets, supermarkets, and shellfishaquacultures in Cilincing and Muara Kamal. Multiple Tube Fermentation (MTF)test, hemolysis test and molecular test for shiga toxin-specific (stx1, stx2), intimin(eaeA) and hemolysin (hlyA) genes have been done. The MTF test results showedthat all samples exceed the threshold of food safety SNI No. 01-2729-3-2006 (>200 MPN/100 g). Hemolysis test results showed that 59,4 % of E. coli isolatedfrom shellfish flesh lysed the red blood cells. The genes responsible for STECexpression were not found in shellfish flesh.;A study was carried out to detect Shiga Toxin-producing Escherichia coli (STEC)in Perna viridis (green mussel) and Anadara granosa (blood cockle) fleshs.Shellfish fleshs were obtained from traditional markets, supermarkets, and shellfishaquacultures in Cilincing and Muara Kamal. Multiple Tube Fermentation (MTF)test, hemolysis test and molecular test for shiga toxin-specific (stx1, stx2), intimin(eaeA) and hemolysin (hlyA) genes have been done. The MTF test results showedthat all samples exceed the threshold of food safety SNI No. 01-2729-3-2006 (>200 MPN/100 g). Hemolysis test results showed that 59,4 % of E. coli isolatedfrom shellfish flesh lysed the red blood cells. The genes responsible for STECexpression were not found in shellfish flesh., A study was carried out to detect Shiga Toxin-producing Escherichia coli (STEC)in Perna viridis (green mussel) and Anadara granosa (blood cockle) fleshs.Shellfish fleshs were obtained from traditional markets, supermarkets, and shellfishaquacultures in Cilincing and Muara Kamal. Multiple Tube Fermentation (MTF)test, hemolysis test and molecular test for shiga toxin-specific (stx1, stx2), intimin(eaeA) and hemolysin (hlyA) genes have been done. The MTF test results showedthat all samples exceed the threshold of food safety SNI No. 01-2729-3-2006 (>200 MPN/100 g). Hemolysis test results showed that 59,4 % of E. coli isolatedfrom shellfish flesh lysed the red blood cells. The genes responsible for STECexpression were not found in shellfish flesh.]