[
ABSTRAKAktivitas bakteri merupakan salah satu penyebab jerawat. Rimpang kencur
(Kaempferia galanga L.) memiliki aktivitas antibakteri yang berasal dari senyawa
etil p-metoksi sinamat (EPMS). Tujuan penelitian ini adalah untuk mengetahui
aktivitas antibakteri dari EPMS ekstrak rimpang kencur terhadap P.acne, S.aureus
dan S.epidermidis serta stabilitas fisik krim dan keamanan krim anti jerawat.
Kristal EPMS diperoleh melalui proses maserasi dengan pelarut heksan, lalu
pemurnian dan identifikasi karakteristik kristal EPMS. Uji aktivitas antibakteri
dan konsentrasi hambat minimum (KHM) pada konsentrasi EPMS 0,3; 0,6; 1,2
dan 2,4% dengan metode difusi cakram dan dilusi cair. Hasil menunjukkan semua
konsentrasi EPMS memiliki aktivitas antibakteri secara signifikan (p<0,01)
dengan zona jernih secara berturut-turut terhadap P.acne (9,00; 11,50; 14,50;
16,00 mm), S.aureus (9,00; 11,50; 16,50; 22,00 mm) dan S.epidermidis (10,50;
12,50; 20,50; 27,00 mm). Senyawa EPMS dengan konsentrasi 0,6; 1,2 dan 2,4 %
terbukti memiliki konsentrasi hambat minimum (KHM) terhadap bakteri P.acne,
sedangkan pada S.aureus dan S.epidermidis pada konsentrasi 1,2 dan 2,4%.
Berdasarkan hasil evaluasi stabilitas fisik, krim EPMS 1,2% memiliki stabilitas
fisik baik hingga akhir penelitian. Dari hasil uji keamanan (pacth test) pada 12
subjek tidak terjadi iritasi alergi, sehingga krim EPMS 1,2% aman digunakan
dalam sediaan topikal.
ABSTRACTAn activity of certain bacterias is one of the causes of acne. Rimpang kencur
(Kaempferiagalanga L.) has an antibacterial agent from compound ethyl pmethoxy
cinnamate (EPMC). The purpose of this research is to find out the
activities of antibacterias using EPMC rimpang kencur extracts against P.acne,
S.aureus and S.epidermidis with the physical stability of the cream and its safety
use as an anti acne cream. The EPMC crystals are obtained through the process of
maceration using hexane solvent, purification and identification of the
characteristic of EPMC crystals. The activity test of the antibacterial and the
minimum inhibitory concentration (MIC) of EPMC are 0,3; 0,6; 1,2 and 2,4% was
done using disk diffusion method and broth dilution test. The result shows that all
EPMC concentration has significant antibanterial activity (p<0,01) respectively
gaining clear zone against P.acne (9,00; 11,50; 14,50; 16,00 mm), S.aureus (9,00;
11,50; 16,50; 22,00 mm) and S.epidermidis (10,50; 12,50; 20,50; 27,00 mm).
EPMC compound with the 0,6; 1,2 dan 2,4 % concentration is proven to have
minimum inhibitory concentration (MIC) against P.acne bacterias, while on the
S.aureus dan S.epidermidis reaches up to 1,2 dan 2,4% concentration. Based on
the results of the evaluation on the physical stability, EPMC 1,2% cream has a
good physical stability until the final research. From the results of safety use
(pacth test) on 12 subjects there were no evidence of allergic irritation, therefore
cream EPMC 1,2% is safe to be used in topical preparation;An activity of certain bacterias is one of the causes of acne. Rimpang kencur
(Kaempferiagalanga L.) has an antibacterial agent from compound ethyl pmethoxy
cinnamate (EPMC). The purpose of this research is to find out the
activities of antibacterias using EPMC rimpang kencur extracts against P.acne,
S.aureus and S.epidermidis with the physical stability of the cream and its safety
use as an anti acne cream. The EPMC crystals are obtained through the process of
maceration using hexane solvent, purification and identification of the
characteristic of EPMC crystals. The activity test of the antibacterial and the
minimum inhibitory concentration (MIC) of EPMC are 0,3; 0,6; 1,2 and 2,4% was
done using disk diffusion method and broth dilution test. The result shows that all
EPMC concentration has significant antibanterial activity (p<0,01) respectively
gaining clear zone against P.acne (9,00; 11,50; 14,50; 16,00 mm), S.aureus (9,00;
11,50; 16,50; 22,00 mm) and S.epidermidis (10,50; 12,50; 20,50; 27,00 mm).
EPMC compound with the 0,6; 1,2 dan 2,4 % concentration is proven to have
minimum inhibitory concentration (MIC) against P.acne bacterias, while on the
S.aureus dan S.epidermidis reaches up to 1,2 dan 2,4% concentration. Based on
the results of the evaluation on the physical stability, EPMC 1,2% cream has a
good physical stability until the final research. From the results of safety use
(pacth test) on 12 subjects there were no evidence of allergic irritation, therefore
cream EPMC 1,2% is safe to be used in topical preparation, An activity of certain bacterias is one of the causes of acne. Rimpang kencur
(Kaempferiagalanga L.) has an antibacterial agent from compound ethyl pmethoxy
cinnamate (EPMC). The purpose of this research is to find out the
activities of antibacterias using EPMC rimpang kencur extracts against P.acne,
S.aureus and S.epidermidis with the physical stability of the cream and its safety
use as an anti acne cream. The EPMC crystals are obtained through the process of
maceration using hexane solvent, purification and identification of the
characteristic of EPMC crystals. The activity test of the antibacterial and the
minimum inhibitory concentration (MIC) of EPMC are 0,3; 0,6; 1,2 and 2,4% was
done using disk diffusion method and broth dilution test. The result shows that all
EPMC concentration has significant antibanterial activity (p<0,01) respectively
gaining clear zone against P.acne (9,00; 11,50; 14,50; 16,00 mm), S.aureus (9,00;
11,50; 16,50; 22,00 mm) and S.epidermidis (10,50; 12,50; 20,50; 27,00 mm).
EPMC compound with the 0,6; 1,2 dan 2,4 % concentration is proven to have
minimum inhibitory concentration (MIC) against P.acne bacterias, while on the
S.aureus dan S.epidermidis reaches up to 1,2 dan 2,4% concentration. Based on
the results of the evaluation on the physical stability, EPMC 1,2% cream has a
good physical stability until the final research. From the results of safety use
(pacth test) on 12 subjects there were no evidence of allergic irritation, therefore
cream EPMC 1,2% is safe to be used in topical preparation]
T41554-Ika Maruya Kusuma.pdf :: Unduh