[ABSTRAK
Nanopartikel emas berpotensi dikembangkan sebagai nano medisin karena sifatnya yang mudah disintesa, mempunyai banyak kegunaan dan biokompatibel padatubuh manusia. Dengan bantuan gom arab sebagai penstabil, vinkristindikonjugasikan dengan nanopartikel emas. Penelitian ini berhasilmengembangkan sintesis dan karakterisasi nanopartikel emas berbasis gom arabterkonjugasi vinkristin dengan distribusi ukuran partikel rata rata dibawah 100 nmdengan menggunakan analisa ukuran partikel dan mikroskop transmisi elektron.Dilakukan uji sitotoksik dengan metode garam tetrazolium/ MTT [3-(4,5-dimethylthiazo-2-yl-)-2,5-diphenyl tetrazolium bromide] pada konjugatvinkristin-gom arab-nanopartikel emas menggunakan lini sel MCF-7 and CCRF.Hasil uji sitotoksisitas digambarkan dengan nilai IC50. Hasil uji pada 2 formula(vinkristin-gom arab-nanopartikel emas sebelum pemurnian dan sesudahpemurnian dengan kromatografi size exclusion) terhadap lini sel MCF-7mempunyai IC50 berturut turut sebesar 3,59 and 3,10 μg/mL, sementaravinkristin murni sebagai pembanding menunjukkan IC50 yang lebih besar yaitu125 μg/mL. Terhadap lini sel CCRF, konjugat sebelum pemurnian dan sesudahpemurnian nilai IC50 berturut turut adalah 1,026 μg/mL dan 2,607 ug/mL.Sementara berdasarkan data in vivo untuk melihat biodsitribusi konjugat padahewan uji dan kemampuan sebagai agen pengontras pada Computed tomography(CT) termyata konjugat mampu terdistribusi di liver dan ginjal hewan uji dandihasilkan nilai Haunsfield Unit (HU) pada uji dengan Computed tomography(CT) pada konjugat VCR-GA-AuNP lebih tinggi dibandingkan nilai HU padaIodium sebagai pembanding. Dari hasil diatas dapat disimpulkan bahwa konjugat(Vinkristin-Gom Arab-Nanopartikel emas) mempunyai kemampuan untuk ditelitilebih lanjut dan dikembangkan sebagai bahan baru terapi obat kanker terarah.;

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ABSTRACT
Gold nanoparticles (AuNP) are potentially developed as nano medicine becauseAuNP are easily synthesized, functionalized, and biocompatible. With gumarabic as stabilizer, vincristine was conjugated with gold nanoparticles. Goldnanoparticles (AuNP) coated with conjugated gum arabic (GA) and vincristine(VCR) were successfully synthesized and characterized. The conjugation of GAVCRand AuNP displayed a narrow hydrodynamic particle size distribution withaverage size < 100 nm by TEM and particle size analyser. We investigatedcytotoxic activity of conjugated vincristine-gum arabic-gold nanoparticle bytetrazolium salt assay (MTT) using cancer cell line MCF-7 and CCRF. Cytotoxicactivity of conjugated VCR-GA-AuNP before and after purification by SizeExclusion Chromatography (SEC), against leukemia cell line CCRF and breastcancer cell line MCF-7 was described by IC50 value. All formulation had acytototoxic of activity with IC50 <20 μg/ml. The IC50 of the conjugate beforeand after purification againts MCF-7 cell line were 3.59 and 3.10 ug/mL,respectively. Meanwhile, the IC50 of vincristine was considerable larger (>125ug/mL). The IC50 of samples againts CCRF cell line were 1,026 μg/mL dan 2,607ug/mL, respectively. Based on in vivo data to evaluate the biodistribution andtheir capacity for a contras agent, conjugate could distributed in the liver andkidneys with the resulting of Hounsfield Unit (HU) value of VCR-GA-AuNP ishigher than the HU value of Iodine as a comparison. In conclusion, conjugatedVCR-GA-AuNP had a good prospect for further investigation and could bedeveloped as materials for new targeted cancer drug therapy;Gold nanoparticles (AuNP) are potentially developed as nano medicine becauseAuNP are easily synthesized, functionalized, and biocompatible. With gumarabic as stabilizer, vincristine was conjugated with gold nanoparticles. Goldnanoparticles (AuNP) coated with conjugated gum arabic (GA) and vincristine(VCR) were successfully synthesized and characterized. The conjugation of GAVCRand AuNP displayed a narrow hydrodynamic particle size distribution withaverage size < 100 nm by TEM and particle size analyser. We investigatedcytotoxic activity of conjugated vincristine-gum arabic-gold nanoparticle bytetrazolium salt assay (MTT) using cancer cell line MCF-7 and CCRF. Cytotoxicactivity of conjugated VCR-GA-AuNP before and after purification by SizeExclusion Chromatography (SEC), against leukemia cell line CCRF and breastcancer cell line MCF-7 was described by IC50 value. All formulation had acytototoxic of activity with IC50 <20 μg/ml. The IC50 of the conjugate beforeand after purification againts MCF-7 cell line were 3.59 and 3.10 ug/mL,respectively. Meanwhile, the IC50 of vincristine was considerable larger (>125ug/mL). The IC50 of samples againts CCRF cell line were 1,026 μg/mL dan 2,607ug/mL, respectively. Based on in vivo data to evaluate the biodistribution andtheir capacity for a contras agent, conjugate could distributed in the liver andkidneys with the resulting of Hounsfield Unit (HU) value of VCR-GA-AuNP ishigher than the HU value of Iodine as a comparison. In conclusion, conjugatedVCR-GA-AuNP had a good prospect for further investigation and could bedeveloped as materials for new targeted cancer drug therapy, Gold nanoparticles (AuNP) are potentially developed as nano medicine becauseAuNP are easily synthesized, functionalized, and biocompatible. With gumarabic as stabilizer, vincristine was conjugated with gold nanoparticles. Goldnanoparticles (AuNP) coated with conjugated gum arabic (GA) and vincristine(VCR) were successfully synthesized and characterized. The conjugation of GAVCRand AuNP displayed a narrow hydrodynamic particle size distribution withaverage size < 100 nm by TEM and particle size analyser. We investigatedcytotoxic activity of conjugated vincristine-gum arabic-gold nanoparticle bytetrazolium salt assay (MTT) using cancer cell line MCF-7 and CCRF. Cytotoxicactivity of conjugated VCR-GA-AuNP before and after purification by SizeExclusion Chromatography (SEC), against leukemia cell line CCRF and breastcancer cell line MCF-7 was described by IC50 value. All formulation had acytototoxic of activity with IC50 <20 μg/ml. The IC50 of the conjugate beforeand after purification againts MCF-7 cell line were 3.59 and 3.10 ug/mL,respectively. Meanwhile, the IC50 of vincristine was considerable larger (>125ug/mL). The IC50 of samples againts CCRF cell line were 1,026 μg/mL dan 2,607ug/mL, respectively. Based on in vivo data to evaluate the biodistribution andtheir capacity for a contras agent, conjugate could distributed in the liver andkidneys with the resulting of Hounsfield Unit (HU) value of VCR-GA-AuNP ishigher than the HU value of Iodine as a comparison. In conclusion, conjugatedVCR-GA-AuNP had a good prospect for further investigation and could bedeveloped as materials for new targeted cancer drug therapy]