UI - Tesis Membership :: Kembali

UI - Tesis Membership :: Kembali

Pengembangan sensor BOD berbasis rhodotorula mucilaginosa UICC Y-181 terimobilisasi pada matriks alginat = Development of a biochemical oxygen demand sensor based on rhodotorula mucilaginosa UICC Y-181 immobilized in alginate matrix

Harmesa; Ivandini Tribidasari Anggraningrum, supervisor; Endang Saefudin, supervisor; Tambunan, Usman Sumo Friend, examiner; Asep Saefumillah, examiner; Yoki Yulizar, examiner; Rahmat Wibowo, examiner (Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2014)

 Abstrak

[ABSTRAK
Elektroda AuNp-BDD dan Au-BDD dikembangkan sebagai elektroda
kerja untuk pengukuran biochemical oxygen demand (BOD) menggunakan
Rhodotorula mucilaginosa UICC Y-181 terimobilisasi pada Na-alginat.
Pengukuran BOD dilakukan dengan mengamati perubahan konsentrasi oksigen
akibat proses oksidasi senyawa organik dalam larutan oleh mikroorganisme.
Glukosa dan galaktosa digunakan sebagai model senyawa organik. Teknik multi
pulse amperometry pada potensial reduksi oksigen -670 mV vs Ag/AgCl
digunakan untuk mendeteksi oksigen sisa yang tidak digunakan oleh
mikroorganisme. Waktu tunggu optimum adalah 10 menit dan ketebalan lapisan
imobilisasi adalah 2 mm. Karakterisasi elektroda dengan XPS menunjukkan rasio
Au/C untuk Au-BDD dan AuNp-BDD adalah 0.0553 dan 0.2084. Pada
pengukuran oksigen, elektroda AuNp-BDD ditemukan lebih sensitif dibandingkan
elektroda Au-BDD maupun elektroda Au. Sedangkan sensor BOD dengan
mikroorganisme terimobilisasi ditemukan memiliki waktu pengukuran lebih cepat,
sensitifitas lebih tinggi, repeatbility lebih baik dan limit deteksi lebih rendah
dibandingkan sensor dengan mikroorganisme bebas. Kelinieran yang baik (R2 =
0.994) ditunjukkan untuk glukosa pada retang konsentrasi 0.1 ? 0.9 mM
(ekuivalen dengan 10 ? 90 mg/L BOD) dan limit deteksi 0.46 mg/L BOD.
Kestabilan arus yang baik ditunjukkan oleh nilai RSD 3.35% (n=8 pengukuran).
Laju konsumsi oksigen dengan sumber karbon glukosa lebih tinggi dari galaktosa
menandakan bahwa khamir Rhodotorula mucilaginosa lebih mudah mendegradasi
glukosa. Keberadaan ion Cu2+ memberikan pengaruh pada sel khamir
Rhodotorula mucilaginosa dengan penurunan konsumsi oksigen.

ABSTRACT
NanoGold-modified boron doped diamond (AuNp-BDD) and Golddeposited
boron doped diamond (Au-BDD) electrodes were used as working
electrodes for measuring biochemical oxygen demand (BOD) using Rhodotorula
mucilaginosa UICC Y-181 immobilized in a Na-alginate matrix. BOD
measurement is done by observing the changes in oxygen concentration due to
oxidation of organic compounds in solution by microorganisms. Glucose and
galactose were used as model organic compounds. Multy-pulse amperometry
technic at -0.67 V (vs Ag/AgCl) of oxygen reduction potensial was used to detect
the remaining of oxygen which is not used by microorganisms. An optimum
waiting time was observed to be 10 min and thickness of immobilization matrix is
2 mm. Ratio of Au/C 0.0553 and 0.2084 was obtained by XPS for Au-BDD and
AuNp-BDD electrodes, respectively. Dissolved oxygen detection, AuNp-BDD
electrode is more sensitive than Au-BDD or Au electrodes. The BOD sensor using
immobilized microorganism was found to have a faster measurement time, higher
sensitivity, better repeatbility and lower detection limit compared to the sensor
using free microorganisms. Good linierity (R2 = 0.994) was shown in the range of
glucose concentration of 0.1 ? 0.9 mM (equivalent to 10 ? 90 mg/L BOD) and
detection limit of 0.46 mg/L BOD. Good stability of currents were shown by an
RSD of 3.35%(n=8). The rate of oxygen consumption to glucose is higher than of
galactose, indicating that glucose is a better substrate for Rhodotorula
mucilaginosa than that of galactose. The presence of Cu ions influence the yeast
cells with a decreasing in oxygen consumption.;NanoGold-modified boron doped diamond (AuNp-BDD) and Golddeposited
boron doped diamond (Au-BDD) electrodes were used as working
electrodes for measuring biochemical oxygen demand (BOD) using Rhodotorula
mucilaginosa UICC Y-181 immobilized in a Na-alginate matrix. BOD
measurement is done by observing the changes in oxygen concentration due to
oxidation of organic compounds in solution by microorganisms. Glucose and
galactose were used as model organic compounds. Multy-pulse amperometry
technic at -0.67 V (vs Ag/AgCl) of oxygen reduction potensial was used to detect
the remaining of oxygen which is not used by microorganisms. An optimum
waiting time was observed to be 10 min and thickness of immobilization matrix is
2 mm. Ratio of Au/C 0.0553 and 0.2084 was obtained by XPS for Au-BDD and
AuNp-BDD electrodes, respectively. Dissolved oxygen detection, AuNp-BDD
electrode is more sensitive than Au-BDD or Au electrodes. The BOD sensor using
immobilized microorganism was found to have a faster measurement time, higher
sensitivity, better repeatbility and lower detection limit compared to the sensor
using free microorganisms. Good linierity (R2 = 0.994) was shown in the range of
glucose concentration of 0.1 ? 0.9 mM (equivalent to 10 ? 90 mg/L BOD) and
detection limit of 0.46 mg/L BOD. Good stability of currents were shown by an
RSD of 3.35%(n=8). The rate of oxygen consumption to glucose is higher than of
galactose, indicating that glucose is a better substrate for Rhodotorula
mucilaginosa than that of galactose. The presence of Cu ions influence the yeast
cells with a decreasing in oxygen consumption., NanoGold-modified boron doped diamond (AuNp-BDD) and Golddeposited
boron doped diamond (Au-BDD) electrodes were used as working
electrodes for measuring biochemical oxygen demand (BOD) using Rhodotorula
mucilaginosa UICC Y-181 immobilized in a Na-alginate matrix. BOD
measurement is done by observing the changes in oxygen concentration due to
oxidation of organic compounds in solution by microorganisms. Glucose and
galactose were used as model organic compounds. Multy-pulse amperometry
technic at -0.67 V (vs Ag/AgCl) of oxygen reduction potensial was used to detect
the remaining of oxygen which is not used by microorganisms. An optimum
waiting time was observed to be 10 min and thickness of immobilization matrix is
2 mm. Ratio of Au/C 0.0553 and 0.2084 was obtained by XPS for Au-BDD and
AuNp-BDD electrodes, respectively. Dissolved oxygen detection, AuNp-BDD
electrode is more sensitive than Au-BDD or Au electrodes. The BOD sensor using
immobilized microorganism was found to have a faster measurement time, higher
sensitivity, better repeatbility and lower detection limit compared to the sensor
using free microorganisms. Good linierity (R2 = 0.994) was shown in the range of
glucose concentration of 0.1 ? 0.9 mM (equivalent to 10 ? 90 mg/L BOD) and
detection limit of 0.46 mg/L BOD. Good stability of currents were shown by an
RSD of 3.35%(n=8). The rate of oxygen consumption to glucose is higher than of
galactose, indicating that glucose is a better substrate for Rhodotorula
mucilaginosa than that of galactose. The presence of Cu ions influence the yeast
cells with a decreasing in oxygen consumption.]

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 Metadata

Jenis Koleksi : UI - Tesis Membership
No. Panggil : T42473
Entri utama-Nama orang :
Entri tambahan-Nama orang :
Entri tambahan-Nama badan :
Program Studi :
Subjek :
Penerbitan : Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2014
Bahasa : ind
Sumber Pengatalogan : LibUI ind rda
Tipe Konten : text
Tipe Media : unmediated ; computer
Tipe Carrier : volume ; online resource
Deskripsi Fisik : x, 96 pages : illustration ; 28 cm + appendix
Naskah Ringkas :
Lembaga Pemilik : Universitas Indonesia
Lokasi : Perpustakaan UI, lantai 3
  • Ketersediaan
  • Ulasan
  • Sampul
No. Panggil No. Barkod Ketersediaan
T42473 15-17-704491372 TERSEDIA
Ulasan:
Tidak ada ulasan pada koleksi ini: 20390259
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