[Tujuan: Menilai keberadaan reseptor PPARγ serta membandingkan tampilan reseptorPPARγ pada endometrium eutopik dan ektopik pada penderita endometriosisMetode: Penelitian ini merupakan penelitian potong lintang (cross sectional). Sepuluhsubjek penderita endometriosis yang menjalani laparoskopi atau laparotomi, yang masukdalam kriteria penerimaan (consecutive sampling) diambil dua percontoh, yakniendometrium eutopik dan endometrium ektopik yang berasal dari dinding kistaendometriosis saat dilakukan pembedahan kemudian dilihat tampilan reseptor PPARγdengan two-step RT-qPCR. Tampilan masing-masing percontoh diuji statistik dengan ujites-t berpasangan dan tes korelasi Pearson.Hasil: Didapatkan tampilan reseptor PPARγ pada endometrium eutopik danendometrium ektopik penderita endometriosis dengan metode RT-qPCR. Tampilanresptor PPARγ endometrium eutopik dan ektopik didapatkan secara statistik tidakberbeda bermakna (1.16 lipatan relatif vs 1.25 lipatan relatif; p=0.26). Pada uji korelasiPesrson didapatkakan korelasi positif lemah antara tampilan PPARγ endometrium eutopikdan ektopik (r=0.16)Kesimpulan: Tampilan reseptor PPARγ pada endometrium eutopik dan ektopikpenderita endometriosis didapatkan dengan metode two-step RT-qPCR. Dengansemikuantifikasi tampilan reseptor PPARγ tidak didapatkan perbedaan antara tampilanreseptor PPARγ pada endometrium eutopik dan ektopik pada penderita endometriosis.Terdapat korelasi positif lemah antara tampilan reseptor PPARγ pada endometriumeutopik dan ektopik pada penderita endometriosis.

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Objective: To evaluate the expression of the PPARγ receptor and to compare its

expression in the eutopic and ectopic endometrium in women with endometriosis

Method: This is a cross sectional study. Ten female subjects with endometriosis that

underwent laparoscopy or laparotomy that fulfilled the inclusion criteria were recruited

by consecutive sampling. Two samples were taken, eutopic endometrium and ectopic

endometrium from endometriosis cyst wall during surgery of each subject, PPARγ

expression was examined by two-step RT-qPCR. Each sample was statistically examined

using the paired t-test and Pearson’s corelation test.

Result: PPARγ was found to be expressed in the eutopic and ectopic endometrium of

women with endometriosis using the RT-qPCR method. The expression of PPARγ was

not statistically different in eutopic and ectopic endometrium (1.16 relative fold vs 1.25

relative fold:p=0.26). By Pearson’s corelation there was a weak positive corelation

between PPARγ expression of the eutopic and ectopic endometrium (r=0.16).

Conclusion: PPARγ was detected by two-step RT-qPCR in eutopic and ectopic

endometrium of women with endometriosis. Semiquantification of PPARγ expression

showed that there was no significant difference betweenits expression in the eutopic and

ectopic endometrium of women with endometriosis. There was a weak postive corelation

of PPARγ expression between the eutopic and ectopic endometrium of women with

endometriosis., Objective: To evaluate the expression of the PPARγ receptor and to compare its

expression in the eutopic and ectopic endometrium in women with endometriosis

Method: This is a cross sectional study. Ten female subjects with endometriosis that

underwent laparoscopy or laparotomy that fulfilled the inclusion criteria were recruited

by consecutive sampling. Two samples were taken, eutopic endometrium and ectopic

endometrium from endometriosis cyst wall during surgery of each subject, PPARγ

expression was examined by two-step RT-qPCR. Each sample was statistically examined

using the paired t-test and Pearson’s corelation test.

Result: PPARγ was found to be expressed in the eutopic and ectopic endometrium of

women with endometriosis using the RT-qPCR method. The expression of PPARγ was

not statistically different in eutopic and ectopic endometrium (1.16 relative fold vs 1.25

relative fold:p=0.26). By Pearson’s corelation there was a weak positive corelation

between PPARγ expression of the eutopic and ectopic endometrium (r=0.16).

Conclusion: PPARγ was detected by two-step RT-qPCR in eutopic and ectopic

endometrium of women with endometriosis. Semiquantification of PPARγ expression

showed that there was no significant difference betweenits expression in the eutopic and

ectopic endometrium of women with endometriosis. There was a weak postive corelation

of PPARγ expression between the eutopic and ectopic endometrium of women with

endometriosis.]