ABSTRAK
Salah satu tanaman obat Indonesia yang berkhasiat sebagai antiinflamasi adalah mengkudu. Tujuan: Mengevaluasi perubahan protein total dan profil protein selHaCaT terinflamasi setelah dipapar ekstrak etanol buah mengkudu. Metode: Sel HaCaT terinflamasi LPS dipaparkan ekstrak etanol buah mengkudu 1%, 10%, dan 40%. Medium kultur dipisahkan dan protein sel diekstraksi menggunakan reagen Trizol. Protein sel dan protein dalam medium kultur diuji Bradford dan profil protein dievaluasi menggunakan metode SDS PAGE. Hasil dan Kesimpulan: Terdapat perubahan konsentrasi protein total dan jumlah pita protein medium dan sel pada semua kelompok perlakuan dan kelompok sel terinflamasi dibanding dengankelompok kontrol.

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ABSTRACT
One of Indonesian herb plant that reported has antiinflammations properties is noni.Objectives: To evaluate the changes of total protein and protein profile in inflamed HaCaT cell after ethanol extract of noni fruit’s exposure. Methods: Inflammed HaCaT cell culture are added by ethanol extract of noni fruits 1%, 10%, and 40%. The culture mediums are separated from the cell while the cell is extracted using Trizol reagent. Then, followed by Bradford assay and analized using SDS PAGE.Result and Conclussion: There are changes on the consentration of total proteins and the protein profile in the medium and cell of inflammed and treatment group compared to the control group.