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ABSTRAKDoksorubisin merupakan obat pilihan utama dalam terapi kanker, tetapi memiliki
indeks terapi rendah. Sehubungan dengan alasan tersebut maka pada penelitian ini
dilakukan pembuatan dan karakterisasi nanopartikel emas (AuNP) - gom arab
terfungsionalisasi doksorubisin untuk meningkatkan indeks terapinya. AuNP
dibuat dengan mereduksi HAuCl4 dengan natrium sitrat kemudian ditambahkan
gom arab sebagai penstabil (GA-AuNP) dan setelah itu difungsionalisasikan
dengan doksorubisin (Dox-GA-AuNP). Dox-GA-AuNP dikarakterisasi dengan
spektrofotometri UV-Vis, spektrofotometri infra merah, dynamic light scattering
dan transmission electron microscopy. Doksorubisin memiliki spektrum serapan
Uv-Vis maksimal pada panjang gelombang 479 nm, sedangkan Dox-GA-AuNP
memiliki spektrum serapan Uv-Vis maksimal pada panjang gelombang 485 nm.
Spektrum IR Dox-GA-AuNP menunjukan adanya pita serapan ikatan keton dan
amin yang berbeda dengan pita serapan ikatan keton dan amin pada doksorubisin
bebas. Ukuran partikel Dox-GA-AuNP adalah 113,6 nm dengan karakteristik
monodispersi (PDI 0,423), dan memiliki morpologi berbentuk sferis. Pengujian
sitotoksik dilakukan terhadap doksorubisin bebas dan Dox-GA-AuNP. Hasil yang
diperoleh menunjukkan bahwa pengujian sitotoksisitas Dox-GA-AuNP pada lini
sel MCF-7 memberikan IC50 0,28 μg/mL sedangkan doksorubisin bebas memiliki
IC50 1,305 μg/mL. Doksorubisin yang telah difungsionalisasikan dengan GAAuNP
dapat mengurangi ikatan protein dengan serum albumin manusia dari 62,51
± 2,21 % (Dox Bebas) menjadi 22,91 ± 10,9 % (Dox-GA-AuNP). Dengan
menurunnya ikatan protein dan meningkatnya efek sitotoksisitas pada Dox-GAAuNP
dibandingkan dengan doksorubisin bebas dapat disimpulkan bahwa Dox-
GA-AuNP dapat meningkatkan indeks terapi doksorubisin.
ABSTRACTDoxorubicin is a drug of choice for cancer therapy, but it has low index therapy.
For that reason the reseach had been done to make and characterize gold
nanoparticle - acacia gum functionalized doxorubicin to increase the therapy
index. Gold nanoparticles was prepared by reducing HAuCl4 with sodium citrate
and gum arabic was added as a steric stabilizer, and then being functionalized by
doxorubicin (Dox-GA-AuNP). Dox-GA-AuNP was characterized by UV - Vis
spectrophotometry, infrared spectrophotometry, dynamic light scattering and
electron microscopy transmission. The UV-Vis spectrometry showed that
doxorubicin has a maximum spectrum absorbtion of 479 nm while Dox-GAAuNP
is 485 nm, FTIR spectrofotometcy showed that Dox-GA-AuNP has ketone
and amine bonds absorption band which is different from absorption band of
doxorubicin. The particle size of Dox-GA-AuNP is 113.6 nm with Poly
Dispersion Index of 0.423, and morphological shape is spheric. The cytotoxic
assay was conducted on MCF-7 cell line for Dox-GA-AuNP and doxorubicin. The
results showed that Dox-GA-AuNP provides IC50 of 0.28 mg / mL while the IC50
of doxorubicin is 1.305 mg / mL. Protein bond of Dox-GA-AuNP is 22.91 ± 10.9
% while protein bond of doxorubicin is 62.51 ± 2.21 %. The decreasing of
protein bond and increasing of cytotoxicity effect of Dox-GA-AuNP compared to
doxorubicin conclude that Dox-GA-AuNP can increase the therapy index of
doxorubicin.;Doxorubicin is a drug of choice for cancer therapy, but it has low index therapy.
For that reason the reseach had been done to make and characterize gold
nanoparticle - acacia gum functionalized doxorubicin to increase the therapy
index. Gold nanoparticles was prepared by reducing HAuCl4 with sodium citrate
and gum arabic was added as a steric stabilizer, and then being functionalized by
doxorubicin (Dox-GA-AuNP). Dox-GA-AuNP was characterized by UV - Vis
spectrophotometry, infrared spectrophotometry, dynamic light scattering and
electron microscopy transmission. The UV-Vis spectrometry showed that
doxorubicin has a maximum spectrum absorbtion of 479 nm while Dox-GAAuNP
is 485 nm, FTIR spectrofotometcy showed that Dox-GA-AuNP has ketone
and amine bonds absorption band which is different from absorption band of
doxorubicin. The particle size of Dox-GA-AuNP is 113.6 nm with Poly
Dispersion Index of 0.423, and morphological shape is spheric. The cytotoxic
assay was conducted on MCF-7 cell line for Dox-GA-AuNP and doxorubicin. The
results showed that Dox-GA-AuNP provides IC50 of 0.28 mg / mL while the IC50
of doxorubicin is 1.305 mg / mL. Protein bond of Dox-GA-AuNP is 22.91 ± 10.9
% while protein bond of doxorubicin is 62.51 ± 2.21 %. The decreasing of
protein bond and increasing of cytotoxicity effect of Dox-GA-AuNP compared to
doxorubicin conclude that Dox-GA-AuNP can increase the therapy index of
doxorubicin., Doxorubicin is a drug of choice for cancer therapy, but it has low index therapy.
For that reason the reseach had been done to make and characterize gold
nanoparticle - acacia gum functionalized doxorubicin to increase the therapy
index. Gold nanoparticles was prepared by reducing HAuCl4 with sodium citrate
and gum arabic was added as a steric stabilizer, and then being functionalized by
doxorubicin (Dox-GA-AuNP). Dox-GA-AuNP was characterized by UV - Vis
spectrophotometry, infrared spectrophotometry, dynamic light scattering and
electron microscopy transmission. The UV-Vis spectrometry showed that
doxorubicin has a maximum spectrum absorbtion of 479 nm while Dox-GAAuNP
is 485 nm, FTIR spectrofotometcy showed that Dox-GA-AuNP has ketone
and amine bonds absorption band which is different from absorption band of
doxorubicin. The particle size of Dox-GA-AuNP is 113.6 nm with Poly
Dispersion Index of 0.423, and morphological shape is spheric. The cytotoxic
assay was conducted on MCF-7 cell line for Dox-GA-AuNP and doxorubicin. The
results showed that Dox-GA-AuNP provides IC50 of 0.28 mg / mL while the IC50
of doxorubicin is 1.305 mg / mL. Protein bond of Dox-GA-AuNP is 22.91 ± 10.9
% while protein bond of doxorubicin is 62.51 ± 2.21 %. The decreasing of
protein bond and increasing of cytotoxicity effect of Dox-GA-AuNP compared to
doxorubicin conclude that Dox-GA-AuNP can increase the therapy index of
doxorubicin.]
T43258-Hadi Sudarjat.pdf :: Unduh