Background: In the Decree of the Minister of Health on the National Immunisation Movement accceleration Universal Child Immunizaton 2010-2014 (GAIN UCI 2010-2014) stated that the general problems of decline in immunization coverage and quality of service were caused by several things, one of which is the lack of quantity, quality and distribution of human resource. Methods: A cross-sectional mix-method study to investige human resouces in vaccine management had been conductited in two provinces in 2012. Primary data were collection by interviewing stakeholders thorughly, secondary data collectin and observation were also carried out. Analysis was done by scoring for data concerning education, length of employment, training experience and knowledge. Result: The number of Vaccine management officers are still lacking. While the immunization guidelines required minimal two offices for for each health centers. Officers' kwoledge in primary health centers is still inadequate, especially in terms of the vaccine and Coldchain (Ice Lined Refrigerators). Ofiicers at Provincial Helath Officehad been trained in vaccine managemnt, but not all officers at District Health Office and Primary Health Centers ha received training yet. Suggestion: This study suggests the addition of the quantity and quality of human resounce in vaccine management, because the office can affect the quality of the vacine, because vassines need a special handling to mainain the quality to provide immunity and Prevent the occurence of diseases that can be prevented by imunization (PD3I).