To implement health decentralization, Ministry of Health has launched Health Ministerial Decree No. 004 Year 2003, stating that in order to strengthen health management in decentralization era there was a need to facilitate health research and development in local government. The study was to conduct a training need assessment for local human resources in health research and development. This TNA study was a case study in East Kalimantan, would samples of Balitbangda Provinsi/Kabupaten/Kota, Dinas Kesehatan, and Bappeda. From 10 basic substances questioned to respondent it has shown most human resources competent falling in the category of fair competence and intermediate competence. If seen from the institution needs above 83% has stated that there was a need for such substances. Seen from availability of training of the substances by the institution above 80% has stated unavailable. From prespective of individual needs above 80% stated that the training was in need by the respondents.