This book constitutes the proceedings of the 12th International Conference on Simulation of Adaptive Behaviour, SAB 2012, held in Odense, Denmark, in August 2012. The 22 full papers as well as 22 poster papers included in this volume were carefully reviewed and selected from 66 submissions. They are organized in topical sections named, animat approach and methodology, perception and motor control, evolution, learning and adaptation, and collective and social behaviour.