[Angka kejadian infeksi dengue tinggi di Indonesia, dan Jakarta untuk lebih
spesifiknya. Virus dengue terdiri dari 4 serotipe, yang serotipe 1 (DENV-1), 2
(DENV-2), 3 (DENV-3), dan 4 (DENV-4). Sampai saat ini, penanganan infeksi
dengue masih mengandalkan diagnosis dini. Meskipun reverse transcription
polymerase chain reaction (RT-PCR) masih sebagai baku emas, metode ini cenderung
mahal dan sulit untuk dilakukan. Inilah alasan untuk pengembangan berbagai tes
cepat, termasuk diantaranya SD Bioline Dengue Duo. Dengan demikian, evaluasi SD
BIOLINE Dengue Duo akan bermanfaat dalam dunia klinis. Peneliti menggunakan
sampel serum yang diambil dari masyarakat, menguji sampel tersebut menggunakan
SD BIOLINE Dengue Duo, dan membandingkannya dengan baku emas [reverse
transcription polymerase chain reaction (RT-PCR) dan isolasi virus pada lini sel
C6/36)]. Ditemukan bahwa sensitivitas, spesifisitas, NPV, dan PPV SD BIOLINE
Dengue Duo untuk mendeteksi demam berdarah serotype campur dan tidak diketahui
adalah 100%. Dalam infeksi DENV-4, SD BIOLINE Dengue Duo menunjukkan
sensitivitas 60% dan spesifisitas 100%., Dengue infection incidence remained high in Indonesia, and Jakarta to be more
specific. Dengue virus consists of 4 serotypes, which are dengue serotype 1 (DENV-1),
2 (DENV-2), 3 (DENV-3), and 4 (DENV-4). Until now, management of dengue infection
still relies on early diagnosis. Although Reverse transcription polymerase chain
reaction (RT-PCR) remains as a gold standard diagnostic method, this method is
costly and difficult to set up. These issues are the reasons for the development of
various rapid tests, including SD Bioline Dengue Duo. Evaluation of SD BIOLINE
Dengue Duo would therefore be beneficial in the clinical setting. Researchers used
serum sample taken from the community, tested the samples using SD BIOLINE
Dengue Duo, and compare it with the gold standard [Reverse Transcription –
Polymerase Chain Reaction (RT-PCR) and virus isolation in cell line C6/36)]. It was
found that the sensitivity, specificity, NPV, and PPV of SD BIOLINE Dengue Duo
for detection of dengue of mixed and unknown serotype were 100%. In infection of
DENV-4, SD BIOLINE Dengue Duo showed a sensitivity of 60% and specificity of
100%.]