UI - Skripsi Membership :: Kembali

UI - Skripsi Membership :: Kembali

Uji efek antioksidan ekstrak air syzygium aromaticum terhadap kerusakan fungsi hati yang diinduksi CCL4 berdasarkan kadar malondialdehida pada tikus wistar = Antioxidant effect of clove (syzygium aromaticum) against CCL4-induced hepatotoxicity in wistar rats indicated by liver malondialdehyde level / Rizqi Amanda Nabilah

Rizqi Amanda Nabilah; Ani Retno Prijanti, supervisor; Ari Estuningtyas, examiner ([Publisher not identified] , 2015)

 Abstrak

ABSTRAK
Pendahuluan: Cengkih (Syzygium aromaticum) mengandung eugenol dipercaya
memiliki efek antioksidan untuk menangkal paparan radikal bebas. Penelitian ini
bertujuan untuk membuktikan efek antioksidan cengkih terhadap kerusakan fungsi hati
tikus Wistar yang diinduksi oleh CCl4 dengan melihat kadar Malondialdehida (MDA)
hati sebagai hasil peroksidasi lipid. Metode: Tiga puluh enam tikus Wistar yang
berusia 12 minggu dibagi menjadi 6 kelompok, yaitu kontrol negatif (CCl4), kontrol
positif (CCl4 + α-tokoferol), dan 4 kelompok dengan pemberian CCl4 dan cengkih
selama 1 hari, 3 hari, 5 hari, dan 7 hari. Hasil: Digunakan uji One-way ANOVA
dengan uji perbandingan post hoc LSD. Didapatkan rerata kadar MDA (nmol/mg
protein) kontrol positif (0.0140), kontrol negatif (0.0098), 1 hari (0.0370), 3 hari
(0.0660), 5 hari (0.0849) dan 7 hari (0.0968). Terdapat perbedaan bermakna (p <0.05)
antar kelompok pada uji One-way ANOVA. Berdasarkan uji post hoc LSD,
peningkatan kadar MDA dibandingkan kontrol negatif signifikan (p<0.05) pada
cengkih 1 hari, 3 hari, 5 hari, dan 7 hari. Kesimpulan: Peningkatan kadar MDA
menandakan adanya peningkatan stress oksidatif pada kelompok yang diberikan
cengkih. Dengan demikian, cengkih bersifat hepatotoksik karena menyebabkan
kerusakan membran lipid.

ABSTRACT
Introduction: Clove (Syzygium aromaticum) contains eugenol as its main compound
known for its antioxidant effect against free radicals. This study was conducted to
investigate the antioxidant effect of Clove against CCl4-induced Wistar rats
hepatotoxicity by measuring liver Malondialdehyde (MDA) level as one of occuring
products of lipid peroxidation. Methods: Thirty–six Wistar rats at the age of 12–
weeks were divided into six groups: positive control (given CCl4 and α-tocopherol),
negative group (CCl4 only), and 4 groups were given CCl4 and clove extract for 1 day,
3 days, 5 days, and 7 days each. Results: One-way ANOVA with post hoc
comparisons (LSD) were performed across all groups. There was a significant
difference (p<0.05) in mean MDA level (nmol/mg protein) between positive control
(0.0140), negative control (0.0098), 1 day of clove (0.0370), 3 days clove (0.0660), 5
days clove (0.0849) and 7 days clove (0.0968). The mean MDA levels are
significantly higher (p<0.05) in groups that were given 1, 3, 5, and 7 days of clove
extract respectively than the negative control group. Conclusions: Higher MDA levels
in clove-given groups indicated increased oxidative stress caused by clove. Therefore,
clove has hepatotoxic effects in Wistar rats instead of antioxidant effects

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 Metadata

Jenis Koleksi : UI - Skripsi Membership
No. Panggil : S-Pdf
Entri utama-Nama orang :
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Entri tambahan-Nama badan :
Program Studi :
Subjek :
Penerbitan : [Place of publication not identified]: [Publisher not identified], 2015
Bahasa : ind
Sumber Pengatalogan : LibUI ind rda
Tipe Konten : text
Tipe Media : computer
Tipe Carrier : online resource
Deskripsi Fisik : xiii, 51 pages : illustration ; 28 cm + appendix
Naskah Ringkas :
Lembaga Pemilik : Universitas Indonesia
Lokasi : Perpustakaan UI, Lantai 3
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