UI - Tesis Membership :: Kembali

UI - Tesis Membership :: Kembali

Optimasi proses nanoenkapsulasi ekstrak minyak cengkeh dengan misel kasein dan uji toksisitas untuk sediaan pestisida nabati = Optimization of nanoencapsulation process of clove oil extract using casein micelle and cytotoxicity assay as plant based pesticide

Teguh Baruji; Muhamad Sahlan, supervisor; Hardaning Pranamuda, supervisor; Heri Hermansyah, examiner; Tania Surya Utami, examiner; Elsa Anisa Krisanti, examiner ([Publisher not identified] , 2015)

 Abstrak

[ABSTRAK
Pestisida nabati berbasis minyak atsiri (essential oils) dapat dijadikan alternatif sebagai
protektan tanaman terhadap serangan hama. Senyawa eugenol dalam minyak atsiri cengkeh
menunjukkan beberapa aktivitas sebagai insektisida, anti jamur dan anti bakteri, namun
bersifat sensitif terhadap degradasi oleh cahaya, panas, oksigen dan masa simpan yang
pendek jika disimpan dalam kondisi tidak tepat. Enkapsulasi senyawa eugenol dirasa sangat
penting untuk melindungi senyawa yang sensitif dari degradasi, menutupi sifat yang tidak
diinginkan dari suatu senyawa, mengurangi kerugian akibat penguapan, mencegah terjadinya
ikatan atau interaksi dengan komponen matriks lainnya dan memfasilitasi pelepasan yang
terkontrol pada kondisi yang diiinginkan sehingga sesuai untuk dijadikan formula pestisida
nabati. Proses optimasi nano enkapsulasi minyak cengkeh menggunakan Response Surface
Methodology (RSM). Central Composite Design (CCD) digunakan untuk mempelajari
pengaruh konsentrasi cengkeh, buffer fosfat dan kalsium klorida terhadap loading capacity
dan efisiensi enkapsulasi produk nano-cengkeh. Persamaan kuadratik terhadap respon
loading capacity didapatkan Y= 60,46+ 7,06A + 6,81B - 0,088 C ?2,26AB + 1,75AC +
2,49BC ? 4,99A2 ? 2,39B2 -2,36C2 , dan terhadap respon efisiensi enkapsulasi Y= 57,65 -
20,67A ? 1,13B + 10,68 C ?5,32AB + 13,59AC + 0,65BC ? 1,00A2 + 11,85B2 -0,50C2.
Optimasi variabel bebas untuk mendapatkan kondisi optimal respon loading capacity dan
efisiensi enkapsulasi berturut-turut sebesar 64,67% dan 79,64% dengan volume cengkeh ,
fosfat dan kalsium masing-masing sebesar 9,82 ; 75 dan 18 ml. Diameter rata-rata
nanocengkeh terbaik yang didapatkan 179,83 nm. Dari uji sitotoksisitas dengan Brine Shrimp
Lethality Test (BSLT) didapatkan data LC50 minyak cengkeh tanpa enkapsulasi sebesar 7,35
ppm (konsentrasi eugenol 4,445 μg/ml) sedangkan sediaan nanocengkeh sebesar 11,50 ppm
(konsentrasi eugenol 0,264 μg/ml ) menunjukkan secara statistik efek enkapsulasi berbeda
sangat signifikan terhadap kematian larva Artemia salina Sp. dibandingkan tanpa enkapsulasi
dengan tingkat kepercayaan 95%.

ABSTRACT
Essential oils based pesticides can be used as an alternative as crop protectant against pests.
Eugenol in clove essential oil showed some activity as insecticides, anti-fungal and antibacterial
but it is sensitive to degradation by light, heat, oxygen and short shelf life when
stored in improper conditions. Encapsulation of eugenol was considered very important to
protect sensitive compound from degradation, covering undesirable properties, reducing
losses due to evaporation, prevent bonding or interaction with other matrix components, and
facilitate the controlled release conditions, so meet the requirement of a plant based
pesticides. Optimization process of nano encapsulation of clove oil extract using Response
Surface Methodology (RSM). Central Composite Design (CCD) employed to study the effect
concentration of clove oil, phospat buffer and calcium chloride on the loading capacity and
encapsulation efficiency of nano-clove product. Quadratic equation of the loading capacity
response was obtained Y= 60,46+ 7,06A + 6,81B - 0,088 C ?2,26AB + 1,75AC + 2,49BC ?
4,99A2 ? 2,39B2 -2,36C2, dan encapsulation efficiency response was obtained Y= 57,65 -
20,67A ? 1,13B + 10,68 C ?5,32AB + 13,59AC + 0,65BC ? 1,00A2 + 11,85B2 -0,50C2.
Optimization of independent variables to obtain optimum conditions loading capacity and
encapsulation efficiency response respectively for 64.67% and 79.64% by volume clove oil,
phospat buffer and calcium chloride respectively 9.82; 75 and 18 ml. The average diameter
of the obtained nano-clove was 179.83 nm. The cytotoxicity assay using Brine Shrimp
Lethality Test (BSLT) LC50 data obtained, clove oil without encapsulation was 7.35 ppm
(eugenol concentration 4.445 μg/ml), while preparations nano-clove was 11.50 ppm (eugenol
concentration 0.264 μg/ml) showed that a statistically encapsulation effects different very
significantly on mortality of larvae Artemia salina Sp. compared without encapsulation with a
confidence level of 95%., Essential oils based pesticides can be used as an alternative as crop protectant against pests.
Eugenol in clove essential oil showed some activity as insecticides, anti-fungal and antibacterial
but it is sensitive to degradation by light, heat, oxygen and short shelf life when
stored in improper conditions. Encapsulation of eugenol was considered very important to
protect sensitive compound from degradation, covering undesirable properties, reducing
losses due to evaporation, prevent bonding or interaction with other matrix components, and
facilitate the controlled release conditions, so meet the requirement of a plant based
pesticides. Optimization process of nano encapsulation of clove oil extract using Response
Surface Methodology (RSM). Central Composite Design (CCD) employed to study the effect
concentration of clove oil, phospat buffer and calcium chloride on the loading capacity and
encapsulation efficiency of nano-clove product. Quadratic equation of the loading capacity
response was obtained Y= 60,46+ 7,06A + 6,81B - 0,088 C –2,26AB + 1,75AC + 2,49BC –
4,99A2 – 2,39B2 -2,36C2, dan encapsulation efficiency response was obtained Y= 57,65 -
20,67A – 1,13B + 10,68 C –5,32AB + 13,59AC + 0,65BC – 1,00A2 + 11,85B2 -0,50C2.
Optimization of independent variables to obtain optimum conditions loading capacity and
encapsulation efficiency response respectively for 64.67% and 79.64% by volume clove oil,
phospat buffer and calcium chloride respectively 9.82; 75 and 18 ml. The average diameter
of the obtained nano-clove was 179.83 nm. The cytotoxicity assay using Brine Shrimp
Lethality Test (BSLT) LC50 data obtained, clove oil without encapsulation was 7.35 ppm
(eugenol concentration 4.445 μg/ml), while preparations nano-clove was 11.50 ppm (eugenol
concentration 0.264 μg/ml) showed that a statistically encapsulation effects different very
significantly on mortality of larvae Artemia salina Sp. compared without encapsulation with a
confidence level of 95%.]

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 Metadata

Jenis Koleksi : UI - Tesis Membership
No. Panggil : T43868
Entri utama-Nama orang :
Entri tambahan-Nama orang :
Entri tambahan-Nama badan :
Program Studi :
Subjek :
Penerbitan : [Place of publication not identified]: [Publisher not identified], 2015
Bahasa : ind
Sumber Pengatalogan : LibUI ind rda
Tipe Konten : text
Tipe Media : unmediated ; computer
Tipe Carrier : volume ; online resource
Deskripsi Fisik : xiii, 82 pages : illustration ; 28 cm + appendix
Naskah Ringkas :
Lembaga Pemilik : Universitas Indonesia
Lokasi : Perpustakaan UI, Lantai 3
  • Ketersediaan
  • Ulasan
  • Sampul
No. Panggil No. Barkod Ketersediaan
T43868 15-18-301973232 TERSEDIA
Ulasan:
Tidak ada ulasan pada koleksi ini: 20414127
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