[
ABSTRAKTujuan dan latar belakang : High grade glioma mecakup hanya 2% dari
seluruh kanker, namun memiliki morbiditas dan mortalitas yang tinggi
walaupun dengan menggunakan pendekatan terapi multimodal menggunakan
kombinasi modalitas operasi, radiasi, kemoterapi dan targetd therapy.
Penelitian ini bertujuan untuk mengetahui korelasi kadar MGMT, sebuah
protein repair, yang diperiksa menggunakan teknik ELISA dengan respon
tumor terhadap radiasi pada High Grade Glioma sehingga diharapkan dapat
menambah pemahaman mengenai sifat biomolekuler dari High grade Glioma.
Metode : Studi ini merupakan sebuah studi restrospektif yang melibatkan 14
pasien yang telah didiagnosa sebagai High Grade Glioma berdasarkan
histopatologi dan telah mendapatkan radiasi postoperasi dengan dan/atau
tanpa chemosensitizer temozolomide di Departemen Radioterapi RSUPN
Cipto Mangunkusumo dari tahun 2004-2015. MGMT diperiksa dengan teknik
ELISA dari jaringan tumor yang sudah diparafinisasi. Respon tumor dihitung
berdasarkan perubahan volume tumor pada imaging CT/MRI pre dan pasca
radiasi.
Hasil: Rerata kada MGMT adalah 184 (160-206) pg/mL. Rerata penyusutan
tumor adalah 10,64% (-75.64-80.20%). Tidak didapatkan korelasi antara
kadar MGMT dengan respon tumor, dengan r= 0.065 (p=0.825). Pada
kelompok yang hanya mendapat radiasi didapatkan r= 0.199 (p=0.607) dan
pada kelompok yang mendapat kemoradiasi dengan TMZ didapatkan korelasi
negatif dengan r= -0,447 (p=0.45).
Kesimpulan : Tidak ada korelasi antara kadar MGMT dengan respon radiasi.
Baik pada kelompok yang mendapatkan radiasi saja ataupun pada kelompok
yang mendapatkan kemoradiasi dengan TMZ.
ABSTRACTPurpose and background : High Grade Glioma comprises just 2% of all
cancer, but it disproportionally has the 6th lowest survival of all cancer found.
Despite combined multimodality approach that has been used by clinician
which can be the combination of two or more modalities of such : surgery,
radiation, chemotherapy and targeted therapy, the mortality and morbidity of
HGG remains high. This study aims to know the correlation between MGMT
protein expression, a repair protein well known in glioma, with the radiation
response, in order to gain more knowledge of the bio molecular behavior of
HGG.
Material and Methods : This study is a retrospective study that involves 14
patients which were diagnosed as HGG based on histopathological findings
and received postoperative radiation with or without concurrent
Temozolomide (TMZ) at the Radiotherapy Department of Cipto
Mangunkusumo Hospital from 2004-2015. Tumor MGMT concentration was
quantified by Enzyme-Linked Immunosorbent Assay from Formalin-Fixed
Paraffin-Embedded (FFPE) tissue. Tumor response was evaluated by
comparing pre and post radiation tumor volume by CT and MRI
Result: MGMT concentration was 184 (160-206) pg/mL. Mean tumor
volume shrinkage was 10,64% (-75.64-80.20%). There were no correlation
between MGMT concentration and tumor response (r= 0.065, p=0.825). The
sample was split according to use of TMZ. In the group that had radiation
only, the correlation between MGMT concentration and tumor response was
not significant (r= 0.199, p=0.607). In the chemoradiation group there was a
moderate negative correlation, but was not significant (r= -0,447, p=0.45).
Conclusion: MGMT protein expression was not correlated with the tumor
radiation response. There was a negative moderate correlation between
MGMT concentrasion and tumor response in patients who underwent
chemoradiation with TMZ, but this correlation was not statistically
significant., Purpose and background : High Grade Glioma comprises just 2% of all
cancer, but it disproportionally has the 6th lowest survival of all cancer found.
Despite combined multimodality approach that has been used by clinician
which can be the combination of two or more modalities of such : surgery,
radiation, chemotherapy and targeted therapy, the mortality and morbidity of
HGG remains high. This study aims to know the correlation between MGMT
protein expression, a repair protein well known in glioma, with the radiation
response, in order to gain more knowledge of the bio molecular behavior of
HGG.
Material and Methods : This study is a retrospective study that involves 14
patients which were diagnosed as HGG based on histopathological findings
and received postoperative radiation with or without concurrent
Temozolomide (TMZ) at the Radiotherapy Department of Cipto
Mangunkusumo Hospital from 2004-2015. Tumor MGMT concentration was
quantified by Enzyme-Linked Immunosorbent Assay from Formalin-Fixed
Paraffin-Embedded (FFPE) tissue. Tumor response was evaluated by
comparing pre and post radiation tumor volume by CT and MRI
Result: MGMT concentration was 184 (160-206) pg/mL. Mean tumor
volume shrinkage was 10,64% (-75.64-80.20%). There were no correlation
between MGMT concentration and tumor response (r= 0.065, p=0.825). The
sample was split according to use of TMZ. In the group that had radiation
only, the correlation between MGMT concentration and tumor response was
not significant (r= 0.199, p=0.607). In the chemoradiation group there was a
moderate negative correlation, but was not significant (r= -0,447, p=0.45).
Conclusion: MGMT protein expression was not correlated with the tumor
radiation response. There was a negative moderate correlation between
MGMT concentrasion and tumor response in patients who underwent
chemoradiation with TMZ, but this correlation was not statistically
significant.]