[Ebi merupakan udang kering berukuran kecil yang dapat dikonsumsi secarakeseluruhan. Ebi diduga memiliki kadar kalsium tinggi meski belum ada penelitianspesifik pada genus tertentu. Penelitian ini bertujuan mengetahui kadar kalsium padaMetapenaeus sp. secara akurat serta menganalisis pengaruh metode preparasi (ovendan non oven) dan metode isolasi (pengocokan, digesti asam, dan pengabuan)terhadap pengukuran dengan ISE dan AAS. Hasil pengukuran didapatkan kadarkalsium tertinggi 6.769 ppm (oven) dan 7.785 ppm (non oven) dengan isolasi terbaikadalah digesti asam. Terdapat perbedaan serta korelasi kadar kalsium antar metodepreparasi dan isolasi, sehingga disimpulkan kedua metode berpengaruh terhadappengukuran kadar kalsium ebi.;Ebi is dried small sized shrimp consumed as a whole. Ebi is assumed to have highcalcium level even there has been no research on specific genus yet. This researchaimed to know exact calcium level in Metapenaeus sp., analyze the influence ofpreparation (oven and non oven) and isolation (dilution, acid digestion, and dryashing) methods toward ISE and AAS measurement. Results showed highest calciumlevel 6,769ppm (oven) and 7,785ppm (non oven) with acid digestion as best isolationmethod. There were differences and correlation among preparation and isolationmethods; and concluded both methods influenced calcium level measurement in ebi., Ebi is dried small sized shrimp consumed as a whole. Ebi is assumed to have highcalcium level even there has been no research on specific genus yet. This researchaimed to know exact calcium level in Metapenaeus sp., analyze the influence ofpreparation (oven and non oven) and isolation (dilution, acid digestion, and dryashing) methods toward ISE and AAS measurement. Results showed highest calciumlevel 6,769ppm (oven) and 7,785ppm (non oven) with acid digestion as best isolationmethod. There were differences and correlation among preparation and isolationmethods; and concluded both methods influenced calcium level measurement in ebi.]