[Penicillium marneffei merupakan fungi patogen yang ditemukan di Asia
Tenggara, khususnya Thailand. Penisiliosis dapat menyebabkan mikosis sistemik
sehingga membahayakan nyawa penderita immunocompromised, khususnya
penderita HIV/AIDS. Antifungi seperti Fluconazole dan Ketoconazole, digunakan
untuk mengatasi infeksi P. marneffei. Akan tetapi, penggunaan antifungi secara
jangka panjang dapat memicu kemungkinan munculnya mutan resisten P.
marneffei. Resistensi pada fungi dapat dipengaruhi beberapa faktor, salah satunya,
overekspresi transporter pengeluaran obat (drug efflux transporter). Mekanisme
pompa pengeluaran obat diatur oleh berbagai transporter. Transporter yang paling
umum diketahui ialah transporter ABC (ATP-binding-cassette) dan MFS (Major
Facilitator Superfamily). Transporter ABC multidrug (MDR) pada P. marneffei
telah dipelajari dengan baik, sedangkan transporter MFS MDR pada fungi
tersebut, belum mendapatkan perhatian yang sama. Penelitian ini fokus pada satu
transporter MFS MDR P. marneffei, yakni PMAA 067100, yang diekspresikan
pada Saccharomyces cerevisiae ADΔ; sistem ekspresi yang sangat rentan terhadap
berbagai macam antifungi. Pengamatan melalui mikroskop konfokal dan uji Disk
Diffusion menunjukkan bahwa transporter PMAA 067100 terlokalisasi pada
membran sel S. cerevisiae ADΔ dan resisten terhadap Fluconazole dan
Terbinafine.;Penicillium marneffei has been known as a pathogenic fungi which is
found in Southeast Asia, especially Thailand. The infection by this fungi
recognized as Penicilliosis, that caused systemic mycosis, might be lethal in
immunocompromised patient, specifically HIV/AIDS patient. Antifungal such as
Fluconazole and Ketoconazole, had been used against P. marneffei infection.
However, the long-term-use of antifungal might cause an emerging resistant strain
of P. marneffei. The resistance phenomenon in fungi is caused by several factors,
one of it is the overexpression of drug efflux transporter. Mechanism of this efflux
pump is regulated by some of transporters such as ABC (ATP-binding-cassette)
and MFS (Major Facilitator Superfamily) transporter. The ABC multidrug (MDR)
transporter of P. marneffei has been studied well, yet the underrated MFS MDR
transporter of the same fungi has not received the same attention. This study focus
on one of P. marneffei MFS MDR transporter, known as PMAA 067100, which
was expressed in Saccharomyces cerevisiae ADΔ; an expression system which is
very susceptible to many kind of antifungal. Observation through confocal
microscope and Disk Diffusion test showed that PMAA 067100 transporter was
localized in S. cerevisiae ADΔ cell membrane and resistant against Fluconazole
and Terbinafine., Penicillium marneffei has been known as a pathogenic fungi which is
found in Southeast Asia, especially Thailand. The infection by this fungi
recognized as Penicilliosis, that caused systemic mycosis, might be lethal in
immunocompromised patient, specifically HIV/AIDS patient. Antifungal such as
Fluconazole and Ketoconazole, had been used against P. marneffei infection.
However, the long-term-use of antifungal might cause an emerging resistant strain
of P. marneffei. The resistance phenomenon in fungi is caused by several factors,
one of it is the overexpression of drug efflux transporter. Mechanism of this efflux
pump is regulated by some of transporters such as ABC (ATP-binding-cassette)
and MFS (Major Facilitator Superfamily) transporter. The ABC multidrug (MDR)
transporter of P. marneffei has been studied well, yet the underrated MFS MDR
transporter of the same fungi has not received the same attention. This study focus
on one of P. marneffei MFS MDR transporter, known as PMAA 067100, which
was expressed in Saccharomyces cerevisiae ADΔ; an expression system which is
very susceptible to many kind of antifungal. Observation through confocal
microscope and Disk Diffusion test showed that PMAA 067100 transporter was
localized in S. cerevisiae ADΔ cell membrane and resistant against Fluconazole
and Terbinafine.]
S62258-Carissa Ista Indriani.pdf :: Unduh