[Infeksi dengue (DENV) merupakan salah satu masalah global yang masih dialami
hingga saat ini. Diperkirakan 50 hingga 100 juta orang di dunia positif mengalami
infeksi dengue.Hingga saat ini, tatalaksana infeksi dengue hanyalah berupa terapi
suportif dan belum ditemukan pengobatan dan vaksin dengue. Swietenia
Mahagoni sudah dikenal dan digunakan sejak dahulu sebagai tanaman obat.
Kandungan flavonoid pada tanaman ini diperkirakan memiliki efek antiviral.
Untuk itu penelitian ini dilakukan untuk melihat potensi antiviral terhadap DENV
dari ekstrak S. Mahagoni. Pada penelitian ini digunakan beberapa konsentrasi
yakni 640 μg/mL, 320 μg/mL, 160 μg/mL, 80 μg/mL, 40 μg/mL, 20 μg/mL, 10
μg/mL dan kontrol negatif adalah DMSO. Untuk menentukan hambatan
infektivitas dapat digunakan metode Focus Assay sehingga dapat diperoleh nilai
Inhibitory Concentration (IC50). Selain itu, untuk dilihat viabilitas sel dengan
metode MTT assay sehingga diperoleh nilai Cytotoxic Concentration (CC50).
Selain itu ditentukan juga nilai indeks selektivitas yang diperoleh dari
perbandingan CC50 dan IC50. Berdasarkan hasil IC50, CC50 dan SI yakni 68,97
μg/mL, 434,46 μg/mL dan 6,29, dapat dikatakan bahwa S. Mahagoni memiliki
efek antiviral sehingga dapat digunakan sebagai antiviral dengue dimasa
mendatang.;Dengue infection (DENV) still becomes as global burden. It is estimated 50 to
100 million people are positively infected by DENV. The recent management of
this disease has not been found yet, with the lack finding of medicine and vaccine,
the main management takes on supportive care. Swietenia mahagoni has been
used as the herbal medicine since long time ago. The flavonoid extract on that
plant is believed to have antiviral effect. This experiment was conducted to
evaluate the antiviral DENV potential of S. mahagoni extract. In this experiment,
the concentration was made on various concentration, from 640 μg/ml, 320
μg/mL, 160 μg/mL, 80 μg/mL, 40 μg/mL, 20 μg/mL, 10 μg/mL, and DMSO as
negative control. Focus assay was used to determine the infectivity inhibition
which results of IC50 (inhibitory concentration), and MTT Assay was used to
determine the cell viability which results of CC50 (cytotoxic concentration).
Selectivity index was also resulted by divided the value of CC50 with IC50. The
results of IC50, CC50, and SI of S. mahagoni was 68,97 μg/mL, 433,46 μg/ml, and
6,29 μg/ml respectively, S. Mahagoni has antiviral effect and could be consider to
be used as antiviral to DENV in the future, Dengue infection (DENV) still becomes as global burden. It is estimated 50 to
100 million people are positively infected by DENV. The recent management of
this disease has not been found yet, with the lack finding of medicine and vaccine,
the main management takes on supportive care. Swietenia mahagoni has been
used as the herbal medicine since long time ago. The flavonoid extract on that
plant is believed to have antiviral effect. This experiment was conducted to
evaluate the antiviral DENV potential of S. mahagoni extract. In this experiment,
the concentration was made on various concentration, from 640 μg/ml, 320
μg/mL, 160 μg/mL, 80 μg/mL, 40 μg/mL, 20 μg/mL, 10 μg/mL, and DMSO as
negative control. Focus assay was used to determine the infectivity inhibition
which results of IC50 (inhibitory concentration), and MTT Assay was used to
determine the cell viability which results of CC50 (cytotoxic concentration).
Selectivity index was also resulted by divided the value of CC50 with IC50. The
results of IC50, CC50, and SI of S. mahagoni was 68,97 μg/mL, 433,46 μg/ml, and
6,29 μg/ml respectively, S. Mahagoni has antiviral effect and could be consider to
be used as antiviral to DENV in the future]
S-Catharina Nenobais.pdf :: Unduh