[Saat ini tengah banyak studi yang dilakukan untuk menemukan antivirus dengue
(DENV) dari ekstrak bebagai tumbuhan sebagai alternatif pengobatan terhadap
infeksi DENV. Calophyllum flavoramulum (C. flavoramulum) merupakan
tanaman yang banyak hidup di daerah Asia Tenggara ini memiliki kandungan dari
turunan xanthone dan flavonoid yang memiliki potensi sebagai antivirus. Pada
studi ini dilakukan evaluasi efek inhibisi dari fraksi etanol ekstrak daun C.
flavoramulum terhadap DENV-2 strain New Guinea C (NGC). Efek inhibisi
(IC50) dievaluasi dengan menggunakan focus assay. Sedangkan efek toksisitas
(CC50) terhadap sel dievaluasi pada sel Huh7it-1 menggunakan metode MTT
assay. Hasil dari studi ini menunjukan CC50 = 473,50ug/ml, IC50 = 41,74ug/ml,
dan SI=11,33. Hasil tersebut menunjukan bahwa C. flavoramulum tidak toksik
terhadap sel hidup dan memiliki nilai SI yang cukup tinggi sehingga efektif untuk
digunakan sebagai antivirus dengue. Akan tetapi, perlu diteliti lebih lanjut
mengenai kandungan aktif dalam C. flavoramulum yang memiliki aktivitas untuk
menghambat replikasi DENV.;In recent years, several studies have been conducted to find dengue (DENV)
antiviral from natural extract as an alternative management for dengue infection.
Calophyllum flavoramulum(C. flavoramulum), one of South East Asia plants,
contains derivates of flavonoid and xanthone where both have been known as
potential dengue antivirals. This study evaluated inhibitory potential of ethanol
extract from C. flavoramulum leaf to DENV-2 strain New Guinea C (NGC)
through focus assay. Along with inhibitory effect (IC50), toxicity effect (CC50) to
Huh7it-1 cell also evaluated through MTT assay. The result of this study shown
IC50 = 41.74 μg/mL, CC50 = 473.50 μg/mL, and SI=11.33. In conclusion, extract
of C. flavoramulum can be used selectively as a dengue antiviral, besides it is not
toxic for living cells. More studies are needed to find its active ingredients which
specifically have the ability to inhibit DENV replication.;In recent years, several studies have been conducted to find dengue (DENV)
antiviral from natural extract as an alternative management for dengue infection.
Calophyllum flavoramulum(C. flavoramulum), one of South East Asia plants,
contains derivates of flavonoid and xanthone where both have been known as
potential dengue antivirals. This study evaluated inhibitory potential of ethanol
extract from C. flavoramulum leaf to DENV-2 strain New Guinea C (NGC)
through focus assay. Along with inhibitory effect (IC50), toxicity effect (CC50) to
Huh7it-1 cell also evaluated through MTT assay. The result of this study shown
IC50 = 41.74 μg/mL, CC50 = 473.50 μg/mL, and SI=11.33. In conclusion, extract
of C. flavoramulum can be used selectively as a dengue antiviral, besides it is not
toxic for living cells. More studies are needed to find its active ingredients which
specifically have the ability to inhibit DENV replication., In recent years, several studies have been conducted to find dengue (DENV)
antiviral from natural extract as an alternative management for dengue infection.
Calophyllum flavoramulum(C. flavoramulum), one of South East Asia plants,
contains derivates of flavonoid and xanthone where both have been known as
potential dengue antivirals. This study evaluated inhibitory potential of ethanol
extract from C. flavoramulum leaf to DENV-2 strain New Guinea C (NGC)
through focus assay. Along with inhibitory effect (IC50), toxicity effect (CC50) to
Huh7it-1 cell also evaluated through MTT assay. The result of this study shown
IC50 = 41.74 μg/mL, CC50 = 473.50 μg/mL, and SI=11.33. In conclusion, extract
of C. flavoramulum can be used selectively as a dengue antiviral, besides it is not
toxic for living cells. More studies are needed to find its active ingredients which
specifically have the ability to inhibit DENV replication.]
Haryo Satrio Muhammad.pdf :: Unduh