ABSTRAKLatar Belakang:
Obesitas merupakan salah satu masalah kesehatan utama yang banyak ditemukan
di negara maju maupun negara berkembang. Obesitas menjadi salah satu faktor
risiko timbulnya penyakit kardiovaskular. Diketahui bahwa populasi obesitas
memiliki kadar plasma BNP yang rendah dibanding kelompok normal. BNP
adalah suatu hormon yang disintesis oleh miosit atrium yang berperan dalam
meregulasi hemodinamik tubuh. Selain itu BNP memiliki efek anti fibrosis dan
anti hipertrofi pada jantung. Dipikirkan bahwa adanya gangguan sintesis BNP di
miosit jantung sebagai salah satu penyebab. Maka, penelitian ini bertujuan untuk
melihat profil ekspresi mRNA BNP, NPR-A dan NPR-C pada populasi obesitas.
Metode:
Studi potong lintang dilakukan di Rumah Sakit Jantung dan Pembuluh Darah
Harapan Kita (RSJPDHK). Jaringan miosit tersimpan yang sudah dilakukan
ekstraksi RNA dibagi menjadi 2 kelompok berdasarkan IMT, kelompok obesitas
(IMT ≥27) dan kelompok normal (IMT <27) dan sesuai kriteria inklusi dan
eksklusi. RNA kedua kelompok dilakukan sintesis cDNA, ekstraksi protein dan
Real-Time PCR untuk mendapatkan mean ΔCt. Kemudian dilakukan
penghitungan menggunakan metode Livak untuk mendapatkan nilai ekspresi
relatif mRNA. Data kemudian di analisis statistik menggunakan SPSS 20.
Hasil Penelitian:
Sebanyak 48 pasien diikutsertakan dalam penelitian ini dengan jumlah kelompok
normal 34 orang dan kelompok obesitas 14 orang. Hasil ekspresi mRNA BNP,
NPR-A dan NPR-C lebih rendah pada kelompok obesitas dibanding kelompok
normal. Namun, tidak didapatkan perbedaan bermakna ekspresi mRNA BNP (p
0,768), NPR-A (p 0,838) dan NPR-C (p 0,768) antara kelompok obesitas
dibanding kelompok normal.
Kesimpulan:
Penelitian ini tidak menemukan perbedaan ekspresi mRNA BNP, NPR-A dan NPR-C yang bermakna antara kelompok obesitas dengan kelompok normal.
ABSTRACTBackground:
Obesity is presenting as a significant health problem across the world. Obesity is a
risk factor for cardiovascular diseases. The plasma level of B-type natriuretic
peptide (BNP) has been identified to be lower in obese people compare to normal.
As we know, BNP is one of the cardiac hormones synthesized by atrial myocyte
that plays a role in hemodynamic regulations. In addition, BNP exerts its anti
fibrotic and anti hypertrophic effects in the heart. It has been hypothesized that
one of the possible mechanism responsible for this inverse relationship is the
impaired synthesize of BNP by cardiomyocytes. Therefore, the aim of our study is
to evaluate the mRNA expression profile of BNP, Natriuretic peptide receptor
type-A (NPR-A) and Natriuretic peptide receptor type-C (NPR-C) in
cardiomyocytes of obese population.
Method:
A cross-sectional study was conducted in Rumah Sakit Jantung dan Pembuluh
Darah Harapan Kita (RSJPDHK). Cardiomyocytes that have been performed the
RNA extraction proses were divided into 2 groups, Obese group (BMI ≥27) and
Normal group (BMI <27), according to BMI and inclusion and exclusion criteria.
Synthesize cDNA, protein extraction and Real-Time PCR were performed in
order to have the mean of ΔCt. Livak method was used to determine the relative
expression mRNA value. SPSS 20 for Windows was used for the purpose of
statistical analyses.
Results:
48 patients were included in this study that consist of 34 patients in normal group
and 14 patients in obese group. The mRNA expression of BNP, NPR-A and NPRC
were
lower in obese group compared to normal group. However, there was no
significant difference between groups.
Conclusion:
In conclusion, there is no significant difference of mRNA expression of BNP, NPR-A and NPR-C between obese and normal group.;Background:
Obesity is presenting as a significant health problem across the world. Obesity is a
risk factor for cardiovascular diseases. The plasma level of B-type natriuretic
peptide (BNP) has been identified to be lower in obese people compare to normal.
As we know, BNP is one of the cardiac hormones synthesized by atrial myocyte
that plays a role in hemodynamic regulations. In addition, BNP exerts its anti
fibrotic and anti hypertrophic effects in the heart. It has been hypothesized that
one of the possible mechanism responsible for this inverse relationship is the
impaired synthesize of BNP by cardiomyocytes. Therefore, the aim of our study is
to evaluate the mRNA expression profile of BNP, Natriuretic peptide receptor
type-A (NPR-A) and Natriuretic peptide receptor type-C (NPR-C) in
cardiomyocytes of obese population.
Method:
A cross-sectional study was conducted in Rumah Sakit Jantung dan Pembuluh
Darah Harapan Kita (RSJPDHK). Cardiomyocytes that have been performed the
RNA extraction proses were divided into 2 groups, Obese group (BMI ≥27) and
Normal group (BMI <27), according to BMI and inclusion and exclusion criteria.
Synthesize cDNA, protein extraction and Real-Time PCR were performed in
order to have the mean of ΔCt. Livak method was used to determine the relative
expression mRNA value. SPSS 20 for Windows was used for the purpose of
statistical analyses.
Results:
48 patients were included in this study that consist of 34 patients in normal group
and 14 patients in obese group. The mRNA expression of BNP, NPR-A and NPRC
were
lower in obese group compared to normal group. However, there was no
significant difference between groups.
Conclusion:
In conclusion, there is no significant difference of mRNA expression of BNP, NPR-A and NPR-C between obese and normal group.
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