ABSTRAK
Latar Belakang: E.faecalis merupakan bakteri saluran akar yang dapat bermigrasi ke jaringan periodonsium pada lesi endo-perio kombinasi murni.Jumlahnya berperan terhadap keparahan penyakit karena sifat virulensinya. Tujuan: Menganalisis tipe strain dan jumlah E.faecalis di saluran akar dan poketlesi endo-perio kombinasi murni, lesi endo primer, dan lesi perio primer. Metode:16 sampel dari 3 kelompok, analisis strain dengan sequencing dan kuantifikasidengan Real Time PCR. Hasil: Terdapat persamaan strain E.faecali,s jumlahterbesar terdapat pada poket lesi endo-perio kombinasi murni. Tidak berbedabermakna antara saluran akar dan poket pada lesi endo-perio kombinasi murni.Kesimpulan; terdapat strain yang sama dan jumlah E.faecalis sebanding antarasaluran akar dan poket lesi endo-perio kombinasi murni.ABSTRACT
Background: E.faecalis is a root canal pathogen that can migrate to periodontal tissue in true combined endo-perio lesion. The quantity of E.faecalis takes part inthe severity of the disease because of its virulence. Objective: To analyze thestrain type and quantity of E.faecalis taken from root canal and pocket of truecombined endo-perio lesion, primary endodontic lesion and primary periodontallesion. Methods: 16 samples were taken from 3 groups, strain type was analyzedby sequencing and quantification by Real Time PCR. Results: There weresimilarities of E. faecalis strain. The largest number were found in the pockets oftrue combined endo-perio lesion. There were no significant differences betweenroot canal and pocket of true combined endo-perio lesions. Conclusion: Therewere similarities strain and quantity of E. faecalis were equal between root canaland pocket true combined endo-perio lesions. ;Background: E.faecalis is a root canal pathogen that can migrate to periodontal tissue in true combined endo-perio lesion. The quantity of E.faecalis takes part inthe severity of the disease because of its virulence. Objective: To analyze thestrain type and quantity of E.faecalis taken from root canal and pocket of truecombined endo-perio lesion, primary endodontic lesion and primary periodontallesion. Methods: 16 samples were taken from 3 groups, strain type was analyzedby sequencing and quantification by Real Time PCR. Results: There weresimilarities of E. faecalis strain. The largest number were found in the pockets oftrue combined endo-perio lesion. There were no significant differences betweenroot canal and pocket of true combined endo-perio lesions. Conclusion: Therewere similarities strain and quantity of E. faecalis were equal between root canaland pocket true combined endo-perio lesions. ;Background: E.faecalis is a root canal pathogen that can migrate to periodontal tissue in true combined endo-perio lesion. The quantity of E.faecalis takes part inthe severity of the disease because of its virulence. Objective: To analyze thestrain type and quantity of E.faecalis taken from root canal and pocket of truecombined endo-perio lesion, primary endodontic lesion and primary periodontallesion. Methods: 16 samples were taken from 3 groups, strain type was analyzedby sequencing and quantification by Real Time PCR. Results: There weresimilarities of E. faecalis strain. The largest number were found in the pockets oftrue combined endo-perio lesion. There were no significant differences betweenroot canal and pocket of true combined endo-perio lesions. Conclusion: Therewere similarities strain and quantity of E. faecalis were equal between root canaland pocket true combined endo-perio lesions. ;Background: E.faecalis is a root canal pathogen that can migrate to periodontal tissue in true combined endo-perio lesion. The quantity of E.faecalis takes part inthe severity of the disease because of its virulence. Objective: To analyze thestrain type and quantity of E.faecalis taken from root canal and pocket of truecombined endo-perio lesion, primary endodontic lesion and primary periodontallesion. Methods: 16 samples were taken from 3 groups, strain type was analyzedby sequencing and quantification by Real Time PCR. Results: There weresimilarities of E. faecalis strain. The largest number were found in the pockets oftrue combined endo-perio lesion. There were no significant differences betweenroot canal and pocket of true combined endo-perio lesions. Conclusion: Therewere similarities strain and quantity of E. faecalis were equal between root canaland pocket true combined endo-perio lesions.