UI - Tugas Akhir :: Kembali

UI - Tugas Akhir :: Kembali

Gambaran klinis diare akut pada anak dan real-time polymerase chain reaction bakteri enteropatogen di Rumah Sakit Cipto Mangunkusumo = Clinical manifestations of pediatric acute diarrhea and real time polymerase chain reaction of bacterial enteropathogen in Cipto Mangunkusumo Hospital

Diana Rahmi; Pramita Gayatri Dwipoerwantoro, supervisor; Nastiti Kaswandani, supervisor (Fakultas Kedokteran Universitas Indonesia, 2016)

 Abstrak

ABSTRAK
Latar belakang: Diare masih merupakan masalah kesehatan masyarakat di
negara berkembang karena morbiditas dan mortalitasnya yang masih tinggi. Diare
dapat disebabkan oleh virus, bakteri dan parasit yang penting diketahui untuk
memberikan tatalaksana yang tepat, namun saat ini belum ada data mengenai
bakteri penyebab diare di Indonesia.
Tujuan: Mengetahui gambaran klinis anak dengan diare akut dan mengetahui
jenis bakteri enteropatogen penyebab diare akut dengan menggunakan real-time
PCR di Rumah Sakit Cipto Mangunkusumo.
Metode: Penelitian potong lintang pada anak dengan diare akut berusia 1-15
tahun di Rumah Sakit Cipto Mangunkusumo.
Hasil: Subyek penelitian ini terdiri dari 60 subyek dengan diare akut. Sebagian
besar berusia 1-3 tahun, status gizi baik, berasal dari ibu dengan pendidikan
sedang dengan status ekonomi keluarga menengah rendah, sebagian besar belum
mendapat antibiotik sebelum ke rumah sakit tetapi sudah mendapat cairan
rehidrasi oral. Gambaran klinis diare akut akibat infeksi bakteri yaitu frekuensi
diare ≤5X/hari (p=0,018), tanpa leukositosis feses (p=0,015) dan malabsorpsi
lemak (p=0,031). Sebaran infeksi bakteri patogen penyebab diare akut
berdasarkan real-time PCR sebagai berikut: Campylobacter jejuni 7 subyek,
Escherichia coli patogen 17 subyek yang terdiri dari EPEC 9 subyek, EIEC 5
subyek dan ETEC 3 subyek. Infeksi bakteri campuran pada subyek sebagai
berikut: EPEC+EIEC 2 subyek , C.jejuni+EPEC 1 subyek, C.jejuni+EPEC+EIEC
1 subyek dan C.jejuni+EPEC+ETEC 1 subyek.
Simpulan: Sebagian besar diare terjadi pada usia 1-3 tahun dengan status pasien
gizi baik dengan status keluarga menengah rendah. Sekitar 48% anak dengan
diare akut didapatkan bakteri dari hasil real-time PCR feses dengan proporsi
terbanyak yaitu EPEC, diikuti Campylobacter jejuni, EIEC dan ETEC.
ABSTRACT
Background: Diarrhea is still a public health problem in developing countries
due to it?s morbidity and mortality. Diarrhea can be caused by viruses, bacteria
and parasites. It is important to know the etiology to provide proper management,
but there is currently no data on the bacteria that causes diarrhea in Indonesia.
Objective: To characterize the clinical manifestations of children with acute
diarrhea and determine the type of enteropathogens bacteria causing acute
diarrhea using real-time PCR in Cipto Mangunkusumo Hospital.
Methods: This was a cross-sectional study, done in June-November 2015. Stool
specimens were collected from patients aged 1-15 years with acute diarrhea and
tested for bacterial enteropathogens using real-time PCR.
Results: Of the 60 children enrolled, mostly aged 1-3 years, good nutritional
status, from low income families and secondary education mothers, most have not
received antibiotics prior to hospital admission but had received oral rehydration
fluids. The clinical features of acute diarrhea caused by bacterial infection is
diarrhea frequency ≤5X / day without fecal leukocytosis and fat malabsorption.
From 60 subjects, 29 (48,3%) children excreted bacteria in their feces prooved by
real-time PCR. Distribution of pathogenic bacterial infection causes acute diarrhea
by real-time PCR as follows: Campylobacter jejuni 7 subjects, pathogenic
Escherichia coli 17 subjects which consists of EPEC 9 subjects, EIEC 5 subjects
and ETEC 3 subjects. Multiple infections in subjects as follows: EPEC+EIEC 2
subjects, EPEC+C.jejuni 1 subject, C.jejuni+EPEC+EIEC 1 subject and
C.jejuni+EPEC+ETEC 1 subject.
Conclusions: Most diarrhea occurs at the age of 1-3 years with good nutritional
status of patients with low-medium family status. Approximately 48% of children
with acute diarrhea excreted bacteria in their feces prooved by real-time PCR
stool with the highest proportion is EPEC, followed by Campylobacter jejuni,
EIEC and ETEC.
;Background: Diarrhea is still a public health problem in developing countries
due to it?s morbidity and mortality. Diarrhea can be caused by viruses, bacteria
and parasites. It is important to know the etiology to provide proper management,
but there is currently no data on the bacteria that causes diarrhea in Indonesia.
Objective: To characterize the clinical manifestations of children with acute
diarrhea and determine the type of enteropathogens bacteria causing acute
diarrhea using real-time PCR in Cipto Mangunkusumo Hospital.
Methods: This was a cross-sectional study, done in June-November 2015. Stool
specimens were collected from patients aged 1-15 years with acute diarrhea and
tested for bacterial enteropathogens using real-time PCR.
Results: Of the 60 children enrolled, mostly aged 1-3 years, good nutritional
status, from low income families and secondary education mothers, most have not
received antibiotics prior to hospital admission but had received oral rehydration
fluids. The clinical features of acute diarrhea caused by bacterial infection is
diarrhea frequency ≤5X / day without fecal leukocytosis and fat malabsorption.
From 60 subjects, 29 (48,3%) children excreted bacteria in their feces prooved by
real-time PCR. Distribution of pathogenic bacterial infection causes acute diarrhea
by real-time PCR as follows: Campylobacter jejuni 7 subjects, pathogenic
Escherichia coli 17 subjects which consists of EPEC 9 subjects, EIEC 5 subjects
and ETEC 3 subjects. Multiple infections in subjects as follows: EPEC+EIEC 2
subjects, EPEC+C.jejuni 1 subject, C.jejuni+EPEC+EIEC 1 subject and
C.jejuni+EPEC+ETEC 1 subject.
Conclusions: Most diarrhea occurs at the age of 1-3 years with good nutritional
status of patients with low-medium family status. Approximately 48% of children
with acute diarrhea excreted bacteria in their feces prooved by real-time PCR
stool with the highest proportion is EPEC, followed by Campylobacter jejuni,
EIEC and ETEC.
;Background: Diarrhea is still a public health problem in developing countries
due to it?s morbidity and mortality. Diarrhea can be caused by viruses, bacteria
and parasites. It is important to know the etiology to provide proper management,
but there is currently no data on the bacteria that causes diarrhea in Indonesia.
Objective: To characterize the clinical manifestations of children with acute
diarrhea and determine the type of enteropathogens bacteria causing acute
diarrhea using real-time PCR in Cipto Mangunkusumo Hospital.
Methods: This was a cross-sectional study, done in June-November 2015. Stool
specimens were collected from patients aged 1-15 years with acute diarrhea and
tested for bacterial enteropathogens using real-time PCR.
Results: Of the 60 children enrolled, mostly aged 1-3 years, good nutritional
status, from low income families and secondary education mothers, most have not
received antibiotics prior to hospital admission but had received oral rehydration
fluids. The clinical features of acute diarrhea caused by bacterial infection is
diarrhea frequency ≤5X / day without fecal leukocytosis and fat malabsorption.
From 60 subjects, 29 (48,3%) children excreted bacteria in their feces prooved by
real-time PCR. Distribution of pathogenic bacterial infection causes acute diarrhea
by real-time PCR as follows: Campylobacter jejuni 7 subjects, pathogenic
Escherichia coli 17 subjects which consists of EPEC 9 subjects, EIEC 5 subjects
and ETEC 3 subjects. Multiple infections in subjects as follows: EPEC+EIEC 2
subjects, EPEC+C.jejuni 1 subject, C.jejuni+EPEC+EIEC 1 subject and
C.jejuni+EPEC+ETEC 1 subject.
Conclusions: Most diarrhea occurs at the age of 1-3 years with good nutritional
status of patients with low-medium family status. Approximately 48% of children
with acute diarrhea excreted bacteria in their feces prooved by real-time PCR
stool with the highest proportion is EPEC, followed by Campylobacter jejuni,
EIEC and ETEC.

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 Metadata

Jenis Koleksi : UI - Tugas Akhir
No. Panggil : SP-PDF
Entri utama-Nama orang :
Entri tambahan-Nama orang :
Entri tambahan-Nama badan :
Program Studi :
Subjek :
Penerbitan : [Place of publication not identified]: Fakultas Kedokteran Universitas Indonesia, 2016
Bahasa : ind
Sumber Pengatalogan : LibUI ind rda
Tipe Konten : text
Tipe Media : computer
Tipe Carrier : online resource
Deskripsi Fisik : xv, 51 pages : illustration + appendix
Naskah Ringkas :
Lembaga Pemilik : Universitas Indonesia
Lokasi : Perpustakaan UI, Lantai 3
  • Ketersediaan
  • Ulasan
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No. Panggil No. Barkod Ketersediaan
SP-PDF 16-18-790356940 TERSEDIA
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Tidak ada ulasan pada koleksi ini: 20424631
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