Diastemas are spaces or gaps between two or more consecutive teeth. They can occur anywhere in the upper or lower dental arches. Diastemas can occur in mixed dentition and closes spontaneously when the canines erupt, but in certain circumstances, diastema will persist until period of permanent teeth. This etiologic factor has to identify because it will influenc effectivenees of closing the multiple diastemas. The multiple diastemas that happened at the anterior upper and lower which caused by deficient tooth structure and missing of some teeth (agenesis), accompanied of central diastema which influenced by anterior labialis frenulum, should treated comprehensively. Treatment of this case entangle ortodontic, periodontic, conservative dentistry, and prostodontic. Reposition of abnormal frenulum attachment (frenectomy), arrangement of occlusion and teeth interdigitation, getting continuity of dental arch through achieving good approximal teeth contact by crown reshaping and replaced of missing tooth element with prothesis, and then making of retainer to maintain stability of treatment result are serial of treatments for the case. By cooperation of multidicipline proffesionals, the final result is to obtain the optimal esthetics, functional, and stability result of treatment, will achived.