ABSTRAK
Telah dilakukan penelitian untuk mengetahui pengaruh variasi konsentrasi molaseterhadap kemampuan Aspergillus flavus UICC 360 dalam menghasilkanlovastatin. Proses fermentasi dilakukan dalam medium Czapek?s Dox Broth(CDB) modifikasi dengan perlakuan variasi konsentrasi molase (0 g/L, 55 g/L, 60g/L, 65 g/L, 70 g/L, 75 g/L, 80 g/L, dan 85 g/L) selama 7 hari pada suhu ruang(27--30˚C) dengan kecepatan agitasi 90 rpm. Ekstraksi senyawa lovastatindilakukan dengan pelarut etil asetat. Pengujian ekstrak lovastatin dilakukandengan metode difusi agar cara cakram terhadap Candida albicans UICC Y-29.Hasil penelitian menunjukkan bahwa nilai rata-rata indeks penghambatan tertinggisebesar 0,49 ± 0,07 diperoleh dari ekstrak lovastatin dengan perlakuan molase 70g/L. Analisis uji Least Significant Difference (LSD) (P < 0,05) menunjukkanbahwa terdapat pengaruh nyata perlakuan konsentrasi molase terhadapkemampuan A. flavus UICC 360 dalam menghasilkan lovastatin. Analisiskualitatif dan kuantitatif lovastatin dengan Kromatografi Cair Kinerja Tinggi(KCKT) menunjukkan keberadaan senyawa lovastatin pada perlakuan molase 70g/L dengan waktu retensi sama dengan lovastatin standar, yaitu 4,5 menit dengankadar 1,1 mg/L.

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ABSTRACT
This research was carried out to determine the effect of concentration variation ofmolasses on the ability of Aspergillus flavus UICC 360 to produce lovastatin. Thefermentation process was carried out using Czapek's Dox Broth (CDB) containingvariation of molasses concentrations (0 g /L, 55 g /L, 60 g/L, 65 g/L, 70 g/L, 75g/L, 80 g/L, and 85 g/L) for 7 days at room temperature (27--30˚C) with agitationspeed of 90 rpm. Extraction of lovastatin was done with ethyl acetate solvent.Lovastatin extracts were tested using agar disc diffusion method against Candidaalbicans UICC Y-29. The result revealed that the highest inhibition index of 0.49± 0.07 was obtained from lovastatin extracts-treated molasses 70 g/L. Analysisusing Least Significant Difference (LSD) (P < 0.05) indicated that there wassignificant difference on the ability of A. flavus UICC 360 to produce lovastatin atdifferent molasses concentration. Qualitative and quantitative analysis oflovastatin using High Performance Liquid Chromatography (HPLC) proved thatlovastatin was present at 70 g/L molasses with the same retention time tolovastatin standard, which was 4.5 minutes, at concentration of 1.1 mg/L.