ABSTRAK
Bioremediasi menggunakan bakteri pendegradasi hidrokarbon, merupakan salahsatu solusi alternatif pengendalian pencemaran tanah oleh senyawa hidrokarbon.Isolat bakteri HL8_5 telah diisolasi dari tanah habitat mangrove. Penelitianbertujuan untuk menganalisis kemampuan biodegradasi senyawa hidrokarbon olehisolat bakteri HL8_5 dan mengetahui karakter fenotipik isolat bakteri. Pengukuranpertumbuhan isolat bakteri HL8_5 dilakukan dengan metode viable plate count,analisis degradasi senyawa hidrokarbon dilakukan dengan gas chromatography/mass spectrometry (GC/MS), dan karakterisasi isolat bakteri dilakukan melaluipengecatan Gram serta perngamatan karakter morfologi dan biokimia. Hasilpenelitian menunjukkan bahwa isolat bakteri HL8_5 mampu tumbuh dalammedium Bushnell-Haas dengan penambahan 1% (v/v) minyak diesel danmengalami peningkatan jumlah populasi bakteri dari 6,98 x 107 CFU/mL hingga1,08 x 1010 CFU/mL setelah inkubasi selama 48 jam. Analisis GC/MSmenunjukkan bahwa senyawa 9-octadecenoic acid mengalami degradasi terbesaroleh isolat bakteri HL8_5 hingga mencapai 40,33% dalam waktu 48 jam, diikutioleh senyawa tetracosane (9,43%) dan tricosane (4,94%). Hasil karakterisasifenotipik menunjukkan bahwa isolat bakteri HL8_5 merupakan bakteri dari familiEnterobacteriaceae.

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ABSTRACT
Bioremediation using hydrocarbon degrading bacteria, is one of the alternativesolutions for handling soil contamination by hydrocarbons. Bacteria isolateHL8_5 has been isolated from soil mangrove habitat. The objective of this studyis to analyze the biodegradation capabilities of bacteria isolate HL8_5 onhydrocarbons and to observe the phenotype characters of bacteria isolate. Growthof bacteria isolate HL8_5 was measured using viable plate count, analysis ofhydrocarbon degradation carried out by gas chromatography/ mass spectrometry(GC/MS), and characterization was done by observing Gram reaction andobservation of morphological and biochemical characters. The results show thatbacteria isolate HL8_5 is able to grow in the Bushnell-Haas medium with additionof 1% (v/v) diesel oil and exhibit increase in the number of bacteria populationfrom 6.98 x 107 CFU/mL to 1.08 x 1010 CFU/mL after 48 hours incubation. TheGC/MS analysis shows that 9-octadecenoic acid has the largest degradation bybacteria isolate HL8_5 up to 40.33% within 48 hours, followed by tetracosane(9.43%) and tricosane (4.94%). The phenotypic characterization indicates thatbacteria isolate HL8_5 is a bacteria from family Enterobacteriaceae.