ABSTRAK
Telah dilakukan penelitian yang bertujuan untuk mengetahui pengaruh pemberianfortifikan Fe Fumarat dalam tepung tempe terhadap kadar zat besi plasma darahtikus (Rattus norvegicus L.) jantan galur Sprague-Dawley. Metode penelitianmenggunakan Rancangan Acak Lengkap (RAL), terdiri atas 25 ekor tikus putihjantan yang dibagi ke dalam 5 kelompok perlakuan, yaitu KK 1 yang diberilarutan CMC 0,5%; KK 2 yang diberi CMC 0,5% dan suspensi tepung tempetanpa fortifikan; dan KP 1, 2, dan 3 yang diberi CMC 0,5% dan tepung tempedengan fortifikan Fe Fumarat dosis 1,35 mg Fe/ kgBB, 2,7 mg Fe/ kg BB, dan 5,4mg Fe/ kgBB selama 21 hari berturut-turut. Pengambilan darah dilakukan padahari ke-0 dan setelah pencekokan hari ke-21. Darah ditentukan kadar zat besinyadengan AAS (Atomic Absorption Spectrophotometer). Hasil uji Anava satu arah(P < 0,05) menunjukkan perbedaan nyata pemberian fortifikan Fe Fumarat dalamtepung tempe terhadap kadar zat besi antar kelompok perlakuan. Peningkatankadar zat besi tertinggi terjadi pada KP 1 yaitu sebesar 13,21% terhadap KK 1 dan11,48% terhadap KK 2.

---

ABSTRACT
The effect of Fe fumarate fortificant addition in tempeh flour on plasma ironconcentration of male Sprague-Dawley rats (Rattus norvegicus L.) had beenstudied. By using Complete Random Design (CRD), twenty five rats were dividedinto five groups, consist of normal control group (KK 1) which was administeredwith CMC 0.5%, treatment control group (KK 2) which was administered withCMC 0.5% and tempeh flour non fortificant, and three treatment groups whichwere administered with tempeh flour added with Fe Fumarate fortificant 1.35 mgFe/KgBW (KP 1); 2.7 mg Fe/KgBW (KP 2); and 5.4 mg Fe/KgBW (KP 3). All ofthe five groups were treated within 21 consecutive days. The plasma ironconcentration was measured by Atomic Absorption Spectrophotometer (AAS).One way ANOVA test ( P < 0.05) showed significant effect of Fe Fumaratefortificant addition in tempeh flour intake on plasma iron concentration in alltreatment groups. The highest increase of plasma iron concentration was detectedon KP 1 which is 13.21% to KK 1 and 11.48% to KK 2.