ABSTRAK
Latar belakang : Gastritis merupakan suatu peradangan pada mukosa lambungsebagai respon terhadap infeksi atau iritasi lambung. Penyebab gastritis kronikyang paling sering adalah infeksi Helicobacter pylori. Adanya Helicobacter pyloriberkaitan dengan terjadinya inflamasi, atropi, serta metaplasia intestinal. BakteriHelicobacter pylori secara morfologi dikenal dengan 2 bentuk yaitu berupa batangdan coccoid. Bakteri yang berbentuk coccoid sulit terdeteksi dengan pewarnaanGiemsa. Untuk itu diperlukan pewarnaan imunohistokimia Helicobacter pyloridan mengukur sensitivitas Helicobacter pylori berbentuk coccoid.Bahan dan metode: Studi potong lintang terhadap 90 jaringan biopsi pasiengastritis kronik pada tahun 2015 dan 2014 yang meliputi 30 kasus Giemsadengan Helicobacter pylori positif, 30 kasus gastritis kronik aktif denganHelicobacter pylori negatif tapi ditemukan bentuk coccoid, dan 30 kasus gastritiskronik non aktif, kemudian dilakukan pewarnaan imunohistokimia Helicobacterpylori.Hasil: Ekspresi Helicobacter pylori bentuk coccoid pada kronik aktif memilikiperbedaan yang bermakna (p<0,05) pada pulasan imunohistokimia. Terdapatperbedaan yang bermakna antara gastritis kronik aktif H.pylori positif danH.pylori negatif pada pulasan IHK dengan derajat inflamasi. Uji sensitivitas danspesifisitas antara pemeriksaan Giemsa dan pulasan imunohistokimia, hasilsensitivitas 65% dan spesifisitasnya 100% .Kesimpulan: Pewarnaan imunohistokimia pada gastritis kronik aktif lebih sensitifdibandingkan dengan pewarnaan Giemsa untuk mendeteksi Helicobacter pyloriterutama jenis coccoid .Kata kunci: gastritis kronik aktif, Giemsa, imunohistokimia Helicobacter pylori

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ABSTRACT
Background: Gastritis is a mucosal inflammation response against infection orgastric irritation. Chronic gastritis most frequently was caused by Helicobacterpylori infection. The presence of Helicobacter pylori was associated withinflammation, atrophy, as well as intestinal metaplasia. Helicobacter pyloribacteria has two morphological form consist of rods and coccoid. Bacteriacoccoid shaped was hard to detect with Giemsa staining. It is necessary toperform immunohistochemical staining to increase the diagnosis sensitivity ofHelicobacter pylori and Helicobacter pylori in coccoid.Materials and Methods: This was a cross sectional study against 90 biopsy ofchronic gastritis years 2015 and 2014 which covers 30 cases of Giemsa stainingwith Helicobacter pylori positive, 30 cases chronic gastritis active withHelicobacter pylori negative but with coccoid form of Helicobacter pylori, and 30cases discovered form coccoid, and 30 cases chronic gastritis non active , thenperformed immunohistochemical staining for Helicobacter pylori.Results: Expression of Helicobacter pylori in active chronic coccoid form has asignificant difference ( p < 0.05 ) on immunohistochemical staining . There is asignificant difference between active chronic gastritis H. pylori- positive and H.pylori- negative staining Immunohistochemistry with the degree of inflammation .The sensitivity and specificity test result between Giemsa examination andimmunohistochemical staining was sensitivity of 65% and specificity of 100 % .Conclusions : Immunohistochemical Staining in active chronic gastritis is moresensitive than Giemsa staining to detect Helicobacter pylori especially in coccoidform.Keywords: Active chronic gastritis, Giemsa, immunohistochemistry Helicobacterpylori