The goal of the research is to produce an application, which can detect malaria on patient through microscopic digital image of blood sample. The research methods are data collection, design analysis, testing and evaluation. The used application methods are image pre-processing, morphology and image segmentation using OpenCV. The expected result is a creation of application, which can be able to detect malaria on a microscopic digital image of patient blood sample. The conclusion is that the application can detect malaria from young trophozoites stadium and gametesocytes from the picture.