Penelitian ini bertujuan untuk mengetahui hubungan faktor-faktor yang dapat mempengaruhi terjadinya kelelahan kerja. Jenis penelitian bersifat deskriptif analitik dengan disain studi cross sectional. Sampel pada penelitian ini berjumlah 58 pekerja di gerbang tol Cililitan PT. Jasa Marga cabang CTC yang dibatasi inklusi dan eksklusi. Pengambilan data dilakukan dengan kuesioner, data sekunder, observasi, dan pengukuran denyut nadi. Hasil penelitian didapatkan 56,9% pekerja mengalami kelelahan tingkat ringan dan 43,1% mengalami kelelahan tingkat sedang. Variabel yang diteliti yaitu durasi kerja, pola shift kerja, beban kerja, waktu istirahat, lama tidur, kondisi kesehatan, dan gambaran workstation. Hasil analisis bivariat didapatkan ada hubungan signifikan antara beban kerja, waktu istirahat, dan lama tidur dengan kelelahan kerja. Disarankan untuk pihak perusahaan melakukan fatigue management guna mencegah dampak dari timbulnya kelelahan.

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This study aims to determine the relationship factors that may influence the occurrence fatigue at work. Types of research is descriptive analytical with cross sectional study design. The sample in this study amounts to 58 workers at the toll collectors Cililitan gate PT. Jasa Marga CTC branch office that restricted inclusion and exclusion. Data were collected by questionnaires, secondary data, observation, and pulse measurement. The results showed that 56.9% of workers experienced low fatigue level and 43.1% experienced moderate fatigue level. The variable are duration of work, work shift patterns, work load, rest periods, length of sleep, health condition, and workstations. Results of bivariate analysis found there is significant relationship between work load, rest periods, and length of sleep with fatigue at work. Recommended for the company is created fatigue management in order to prevent the impact of the onset of fatigue.