Majalah, Jurnal, Buletin :: Kembali

Majalah, Jurnal, Buletin :: Kembali

The southeast asian journal of tropical medicine and public health

(Official publicaation of the SEAMEO regional tropical medicine and public health network (TROPMED) , [Date of publication not identified] )
 Abstrak
This study aimed to demonstrate class switch recombination (CSR) in heavy chain expressing immunoglobulin G (IgG) and IgE in human B cells (CD20+CD27-) were cultured with crude P. falciparum antigen (cPfAg) and anti-CD40. on day 4 post-exposure, total RNA from B cells was prepared and the occurance of CSR from IgM to IgG and/or IgE was investigated by reverse transciption-polymerase chain reaction. Molecular markers to detect active CSR included enzyme activation-induced cytidine deaminase mRNA, y and e-germline transcripts (y,e-GLT), circle transcript (CT) and mature transcript (y and e-mRNA) expression. On day 7 and day 14 after exposure, levels of Igs in the culture supernatant were determined by enzyme-linked immunosorbent assay. Our findings showed that we could demonstrate cPfAg-stimulated B cells undergoing CSR by use of the expressed CSR markers and the increase in spesific IgG and IgE indicating the potential of this approach in the study of CSR in P. falciparum stimulated B cells.
 Metadata
No. Panggil : 610 TSAJ
Subjek :
Penerbitan : Bangkok, Thailand: Official publicaation of the SEAMEO regional tropical medicine and public health network (TROPMED) , [Date of publication not identified]
Sumber Pengatalogan LibUI eng rda
Tipe Konten teks
Tipe Media unmediated
Tipe Carrier volume (rdacarier)
Frekuensi Terbit
Kepemilikan [610 TSAJ 40:4 (2009)]
Alamat Penerbit Faculty of Tropical Medicine, Mahidol University 420/6 Ratchawithi Road, Bangkok 10400, Thailand.
Lembaga Pemilik Universitas Indonesia
Lokasi Perpustakaan UI, Lantai 4, R. Koleksi Jurnal
  • Ketersediaan
  • Ulasan
  • Sampul
No. Panggil No. Barkod Ketersediaan
610 TSAJ TERSEDIA
Ulasan:
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