Olahan buah nanas merupakan salah satu komoditas buah tropis terbesar produksinya di Indonesia yang berpotensi menghasilkan limbah berupa kulit dan bonggol. Penelitian ini bertujuan mengisolasi bromelain dari ekstrak bonggol nanas Ananas comusus [L]. Merr yang dimurnikan melalui metode fraksionasi bertingkat dengan etanol. Fraksi bromelain yang diperoleh kemudian aktivitas antiagregasi plateletnya di uji secara in vivo menggunakan mencit putih jantan galur ddy dengan asetosal sebagi control positif. Fraksinasi enzim kasar bromelain dengan etanol menghasilkan aktifitas spesifik tertinggi pada fraksi etanol 30-60 fraksi 2 yaitu sebesar 3,107 Unit/g dengan tingkat kemurnian 155 kali dari enzim kasarnya Pada uji antiagregasi platelet menunjukkan bahwa fraksi enzim bromelain dengan dosis 70 g/Kg bb, 140 g/Kg bb, dan 210 g/Kg bb dapat meningkatkan waktu pendarahan, waktu koagulasi secara bermakna.

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Processed pineapple is one of largest commodities tropical fruit production in Indonesia and it will bring waste from the skin and core .This study aims to isolate bromelain of an extract core the pineapple Ananas comusus L .Merr are purified by fractionation using ethanol and continued by test activity as an antiplatelets agent by in vivo method using white mice male ddy type with asetosal as positive control. Fractionation of crude enzyme bromelain with ethanol produces highest specific activity on ethanol 30 60 faction faction 2 3,107 Unit mg and the protein content 61, 25 mg with the degree of purity of 155 times of crude enzyme. In antiplatelet aggregation tests shows that faction an enzyme bromelain with doses 70 g Kg bb, 140 g Kg bb, and 210 g Kg bb can increase time bleeding , time coagulation meaningful p 0.05 , while in test anti aggregation of platelets by measuring absorption plasma based on a method of born the aggregator ADP , isolates an enzyme with doses 70 g Kg bb having the percent value inhibition aggregation of platelets greater and consistent every week by the provision of doses than 140 g Kg bb, and 210 g Kg bb.