The Durian Tengkurak (Durio tanjungpurensis Navia) is one of the endangered exotic species in the Malvaceae family.
The species is important for conservation of the germplasm and is considered a potential genetic resource for the
development of durian in the future. The objective of this research project was to assess the molecular diversity of D.
tanjungpurensis in West Kalimantan, based on Inter Simple Sequence Repeat (ISSR) markers. We applied ten ISSR
primers to reveal the genetic diversity of 60 individuals from six natural endemic D. tanjungpurensis populations. The
genetic diversity parameters were estimated based on binary data about PCR products (present or absent bands). The
results showed that the mean number of observed alleles, the mean number of effective alleles, the genetic diversity, the
Shannon’s Information Index score, the number of polymorphic loci, and the percentage of polymorphic loci were 1.53,
1.29, 0.17, 0.26, 77.83, and 52.59, respectively. An analysis of molecular variance (AMOVA) showed that the genetic
diversity within a population (65%) was higher than that found between the populations (35%). UPGMA clustering and
principal coordinate analysis, based on the DICE similarity matrix, were used to classify the populations into three
groups: 1) Hutan Rejunak and Tembaga, 2) Bukit Merindang, and 3) Hutan Rawak, Bukit Sagu 1, and Bukit Sagu 2.
Further analysis of the population structure using STRUCTURE software was used to classify all the individuals into
two major categories, thus uniting Groups 2 and 3 as one major category. In conclusion, a high level of genetic diversity
in the Durian Tengkurak was revealed utilizing the ISSR markers employed in the study.
Diversitas Molekuler Berbasis ISSR pada Durio tanjungpurensis Asal Kalimantan Barat, Indonesia. Durian
Tengkurak (Durio tanjungpurensis Navia) adalah salah satu spesies langka yang eksotis dari suku Malvaceae. Durian
tersebut bernilai penting untuk konservasi plasma nutfah dan berpotensi sebagai sumber daya genetik untuk
pengembangan durian di masa depan. Tujuan penelitian adalah mengetahui keragaman molekuler D. tanjungpurensis
asal Kalimantan Barat berdasarkan penanda Inter Simple Sequence Repeat (ISSR). Sepuluh primer ISSR digunakan
untuk mengetahui keragaman genetik 60 individu Durian Tengkurak dari enam populasi endemik alami D.
tanjungpurensis. Parameter keragaman genetik didasarkan pada data biner pita DNA produk PCR, yaitu ada atau tidakada
pita. Hasil penelitian menunjukkan bahwa rata-rata jumlah alel, rata-rata jumlah efektif alel, diversitas genetik,
indeks informasi Shannon, jumlah polimorfik lokus, dan persentase polimorfik lokus berturut-turut adalah 1,53, 1,29,
0,17, 0,26, 77,83 dan 52,59. Analisis ragam molekuler (AMOVA) menunjukkan keragaman genetik yang lebih tinggi di
dalam populasi (65%) dibandingkan antar populasi (35%). Analisis gugus menggunakan metode UPGMA berdasarkan
matriks keserupaan Dice dan analisis koordinat utama digunakan untuk mengelompokkan semua individu populasi ke
dalam tiga kelompok, yaitu grup 1 (Hutan Rejunak dan Tembaga), grup 2 (Bukit Merindang), dan grup 3 (Hutan
Rawak, Bukit Sagu 1 dan Bukit Sagu 2). Analisis lebih lanjut terhadap struktur populasi menggunakan program
STRUCTURE menyatukan grup 2 dan 3 ke dalam satu grup utama. Penelitian ini berhasil mengungkap keragaman
genetik Durian Tengkurak menggunakan penanda ISSR.
