ABSTRAK
Ruang Lingkup dan Cara Penelitin : Perubahan glikoproteinmembrari sel sering dihubungkan dengan transformasikeganasan. Salah satu komponen karbohidrat glikoprotein membren adalah L?fukosa. . Diketahui bahwa kadar L?fukosa glikoprotein dalam serum penderita berbagaikeganasan lebih tinçgi dibandinçkan dengan normal.Jika peningkatan kadar fukosa glikoprotein terjadicukup dini, penentuannya mungkin dapat menuniukkan awalperubahan ke arah keganasan. Untuk nengetahui apakahpeningkatan kadar luko?a serum terjadi pada awal peru?bahan ke arch keganasan hati, maka diteliti bagaimanahubungan perubahan kadar fukosa serum dan gambaranmikroskopis jaringan hati tikus yang diberi aflatoksinB1 (AFB1) untuk menginduksi kanker. Dalam penelitianini digunakan 36 tikus diberikan AFB1. dari 36 tikusdlberikan pelarut Wb1. Aflatoksin dengan pelarutdimetilformamida diberikan secara inkubasi lambung 20ug, sekali 2 hari selama 12 minggu. Pengambilan serumdari hati tikus dilakukan, setiap 4 minggu mulai mingguke?8 sampai minggu ke?40. Penentuan kadar fukosa serumdilakuken sesuai dengan metode yang dilakukan olehWinzler dan sediaan jaringan hati dibuat dengan pewarnaan HE menurut Bhomer.Hasil dan Kesjmpulan: Hasjl penelitian menunjukkan, bahwakenaikan kadar fukosa glikoprotein serum tikus yangdiberi AFB. lebih tinggi secara bermakna dibandingkandengan kontrol. Kadar rata?rata fukosa serum tikusyang diberi AFB1 seluruh pengamatan adalah 13,09 ± 0,99mgflOO mL dan tikus kontrel cdalah 11,72 ± 0,84 mg/lOOmL. Disamping itu ternyata ada kecenderungan peningkatan kadar lukosa serum sejalan dengan waktu, namunkenaikan peningkatan ini secara statistik belum bermakna untuk pengukuran pada waktu pengamatan yang sama.Jaringan hati tikus yang diberi AFB1, memperlihatkangambaran, hiperplasia saluran empedu (perubahan awal kearah keganasan) mulai pengamatan minggu ke?36. Darihasil penelitian ini: dapat disimpulkan, bahwa pemberianAFBJ menyebabkan peningkatan kadar lukosa serum, namunpada penelitian ini belum depat dinyatakan perbedaanyang bermakna pada seat sel menunjukkan perubahan awalke arah keganasan.

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ABSTRACT
Scope and Method of Study: Cell membrane glycoproteinchanges is often related to malignant transformation.One of the carbohydrate components of membrane glycoprotein, often observed to be increased in malignancies, is L?fucose. If this increase appears earlyduring the formation of malignancies it could be usedas a marker. The aim of this study was to determinewhether an increase of serum fucose concentration canbe detected during early malignant transformation.Thirty six rats were administrated aflatoxin B1 indimethylformamide by gastric intubation and 36 ratswere used as controls. Aflatoxin was given in 20 ugdosages every two days for a period of 12 weeks. Serumand liver samples were collected once every 4 weeksfrom week?8 until week?40. Serum fucose concentrationmeasured by the method introduced by Winzler analiver slices were stained with hematoxylin and .eosinaccording to Bhomer.Findings and conclusions: The serum protein?bound fucoseconcentration in rats treated with aflatoxin B1, wassignificantly higher than in the control group. Themean concentration in the treated rats was 13.09 ± 0.99mg/l00 mL and in the control group was 11.72 ± 0.84mg/l00 mL. There was also a positive correlationbetween the increase of serum fucose concentration andthe time of observation, although the increase in bothgroups measured concurrently did not show significantdifferences. The aflatoxin B1 treated rats show bileduct hyperplasja (known as one of the earliest apparentpreneoplastic changes) as early as the 36th week. Itcan be concluded that the administration of aflatoxin8 causes increased serum fucose levels, although inthis study at the onset of cell differentiation towardmalignancy, this difference was still not significant.